Effects of Qing Hua Chang Yin on lipopolysaccharideinduced intestinal epithelial tight junction injury in Caco2 cells.
Mol Med Rep
; 23(3)2021 03.
Article
en En
| MEDLINE
| ID: mdl-33495820
ABSTRACT
Disruption of the intestinal mucosal barrier integrity is a pathogenic process in inflammatory bowel disease (IBD) development, and is therefore considered a drug discovery target for IBD. The wellknown traditional Chinese formulation Qing Hua Chang Yin (QHCY) has been suggested as a potential therapeutic agent for the treatment of ulcerative colitis. However, the possible underlying molecular mechanisms regarding its therapeutic effect remain unclear. Consequently, the present study investigated the effects of QHCY on lipopolysaccharide (LPS)induced loss of intestinal epithelial barrier integrity in vitro using the Caco2 cell model of intestinal epithelium. QHCY reversed the LPSinduced decrease in transepithelial electrical resistance and significantly alleviated the increased fluorescentlylabeled dextran 4 flux caused by LPS. Moreover, QHCY upregulated the mRNA and protein expression levels of occludin, zona occludens1 and claudin1 in LPSexposed Caco2 cells. In conclusion, QHCY was able to protect intestinal epithelial barrier integrity following an inflammatory insult; the protective effects of QHCY may be mediated by modulation of the expression of tight junction proteins.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Medicamentos Herbarios Chinos
/
Lipopolisacáridos
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Uniones Estrechas
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Células Epiteliales
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Mucosa Intestinal
Límite:
Humans
Idioma:
En
Revista:
Mol Med Rep
Año:
2021
Tipo del documento:
Article