Evaluation of the potential anticancer activity of different vitamin D metabolites on colorectal and breast cancer cell lines.
Horm Mol Biol Clin Investig
; 42(1): 3-9, 2021 Feb 01.
Article
en En
| MEDLINE
| ID: mdl-33544505
ABSTRACT
OBJECTIVES:
Vitamin D is very important for calcium and mineral metabolism, and many hypotheses appear to link sunlight exposure with cancer risk and prognosis. As many studies supported the antitumor effect of vitamin D we wanted to investigate the potential effect of multiple vitamin D metabolites.METHODS:
This study compared the anticancer effect of three inactive forms of vitamin D3 which are; cholecalciferol, alfacalcidol, and calcifediol on two human cancer cell lines colorectal cancer (CaCo II) and breast cancer (MCF-7). All were examined after 24, 48, and 72 h continuous exposure using a colorimetric assay (MTT) seeded in 96-multiwell plates. Doxorubicin anticancer used as a standard agent for comparison, while normal skin fibroblast cells (HDFa) was used as our negative control. IC50 values were calculated as indication of antitumor effect.RESULTS:
Broad-spectrum of cytotoxicity with IC50 values ranging from 4 to 200 µM were found. Alfacalcidol was the most potent cytotoxic agents on colorectal cancer (CaCo II) and breast cancer (MCF-7) compared to cholecalciferol, and calcifediol. Both, alfacalcidol and calcifediol were more cytotoxic than cholecalciferol on the tested cell lines as they are partially active metabolites. Breast cancer (MCF-7) was the most sensitive to all metabolites at all-time intervals with the best IC50 values of 4.35 µM ± 1.06 after 72 h continuous exposure of alfacalcidol.CONCLUSIONS:
Vitamin D metabolites are a potential option for cancer treatment along with or an alternative to chemo-therapeutics although extensive preclinical studies are required to prove this effect.Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Contexto en salud:
6_ODS3_enfermedades_notrasmisibles
Problema de salud:
6_breast_cancer
/
6_colon_rectum_cancers
Asunto principal:
Vitamina D
/
Neoplasias de la Mama
/
Calcifediol
/
Neoplasias Colorrectales
/
Colecalciferol
/
Hidroxicolecalciferoles
/
Antineoplásicos
Límite:
Female
/
Humans
Idioma:
En
Revista:
Horm Mol Biol Clin Investig
Año:
2021
Tipo del documento:
Article
País de afiliación:
Jordania