Sleep Deprivation Interferes with JAK/STAT Signaling Pathway and Myogenesis in the Masseter Muscle of Rats.
Med Princ Pract
; 30(3): 253-261, 2021.
Article
en En
| MEDLINE
| ID: mdl-33601387
ABSTRACT
OBJECTIVES:
The aim of the study was to study the Janus kinase/tyrosine kinase-activated transduction factor (JAK/STAT) signaling pathway and myogenesis on the masseter muscle after sleep deprivation and to investigate the role of stress in this scenario. SUBJECTS ANDMETHODS:
A total of 18 male Wistar rats were divided into the following groups control (n = 6) animals were not submitted to any procedures, and paradoxical sleep deprivation and vehicle (PSD + V; n = 6) animals were subjected to PSD for 96 h and (PSD + MET; n = 6) animals were subjected to PSD for 96 h with administration of metyrapone. Paradoxical sleep deprivation was performed by the modified multiple platforms method. Histopathological analysis, histomorphometry, and immunohistochemistry were performed.RESULTS:
The results showed the presence of inflammatory infiltrate in the PSD + V and PSD + MET groups and atrophy. Histomorphometry showed that the cellular profile area decreased, while cellular density increased in both experimental groups. Expression of p-STAT 3, MyoD, and MyoG increased in the PSD + V group, while the PSD + MET group showed increased expression of IL-6 and p-STAT 3.CONCLUSION:
Our results suggest that sleep deprivation induces an inflammatory response and atrophy in the masseter muscle of rats.Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Atrofia
/
Privación de Sueño
/
Proteínas Tirosina Quinasas
/
Atrofia Muscular
/
Desarrollo de Músculos
/
Quinasas Janus
/
Músculo Masetero
Límite:
Animals
Idioma:
En
Revista:
Med Princ Pract
Asunto de la revista:
EDUCACAO
Año:
2021
Tipo del documento:
Article
País de afiliación:
Brasil