Radio frequency heating improves water retention of pork myofibrillar protein gel: An analysis from water distribution and structure.

ABSTRACT

This study was to explore effects of hot air assisted or not assisted radio frequency (RF, 27.12 MHz, 1.4 kW) heating with different electrode gaps (100 mm, 120 mm, and 140 mm) on the water-holding capacity (WHC) of myofibrillar protein (MP) gel and to understand the underlying mechanism through chemical forces, water distribution, and structure. The results showed that the MP gels heated by RF (100 mm) had the highest WHC and uniform gel network structure. As for RF with 100 mm electrode gap, the increased ionic and hydrogen bonds might be conducive to the WHC compared to water bath heating, which was verified by Low-field nuclear magnetic resonance results that the free water converted into the immobilized water. Raman spectroscopy results revealed that RF (100 mm) induced the self-assembly of ß-sheet to α-helix, which conduced to the stable and ordered gel network structure.