Characterization of a GlgC homolog from extremely halophilic archaeon Haloarcula japonica.
Biosci Biotechnol Biochem
; 85(6): 1441-1447, 2021 May 25.
Article
en En
| MEDLINE
| ID: mdl-33749776
Glycogen synthesis in bacteria is mainly organized by the products of glgB, glgC, and glgA genes comprising the widely known glg operon. On the genome of extremely halophilic archaeon Haloarcula japonica, there was a gene cluster analogous to the bacterial glg operon. In this study, we focused on a GlgC homolog of Ha. japonica, and its recombinant enzyme was prepared and characterized. The enzyme showed highest activity toward GTP and glucose-1-phosphate as substrates in the presence of 2.6 m KCl and predicted to be work as "GDP-glucose pyrophosphorylase" in Ha. japonica.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Homología de Secuencia de Ácido Nucleico
/
Haloarcula
/
Proteínas Arqueales
Idioma:
En
Revista:
Biosci Biotechnol Biochem
Asunto de la revista:
BIOQUIMICA
/
BIOTECNOLOGIA
Año:
2021
Tipo del documento:
Article
País de afiliación:
Japón