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Early-stage dynamics of chloride ion-pumping rhodopsin revealed by a femtosecond X-ray laser.
Yun, Ji-Hye; Li, Xuanxuan; Yue, Jianing; Park, Jae-Hyun; Jin, Zeyu; Li, Chufeng; Hu, Hao; Shi, Yingchen; Pandey, Suraj; Carbajo, Sergio; Boutet, Sébastien; Hunter, Mark S; Liang, Mengning; Sierra, Raymond G; Lane, Thomas J; Zhou, Liang; Weierstall, Uwe; Zatsepin, Nadia A; Ohki, Mio; Tame, Jeremy R H; Park, Sam-Yong; Spence, John C H; Zhang, Wenkai; Schmidt, Marius; Lee, Weontae; Liu, Haiguang.
Afiliación
  • Yun JH; Department of Biochemistry, College of Life Sciences and Biotechnology, Yonsei University, Seodaemun-gu, 120-749 Seoul, South Korea.
  • Li X; Complex Systems Division, Beijing Computational Science Research Center, Haidian, 100193 Beijing, People's Republic of China.
  • Yue J; Department of Engineering Physics, Tsinghua University, 100086 Beijing, People's Republic of China.
  • Park JH; Department of Physics, Beijing Normal University, Haidian, 100875 Beijing, People's Republic of China.
  • Jin Z; Department of Biochemistry, College of Life Sciences and Biotechnology, Yonsei University, Seodaemun-gu, 120-749 Seoul, South Korea.
  • Li C; Department of Biochemistry, College of Life Sciences and Biotechnology, Yonsei University, Seodaemun-gu, 120-749 Seoul, South Korea.
  • Hu H; Department of Physics, Arizona State University, Tempe, AZ 85287.
  • Shi Y; Department of Physics, Arizona State University, Tempe, AZ 85287.
  • Pandey S; Complex Systems Division, Beijing Computational Science Research Center, Haidian, 100193 Beijing, People's Republic of China.
  • Carbajo S; Department of Engineering Physics, Tsinghua University, 100086 Beijing, People's Republic of China.
  • Boutet S; Physics Department, University of Wisconsin, Milwaukee, Milwaukee, WI 53201.
  • Hunter MS; Linac Coherent Light Source, Stanford Linear Accelerator Center National Accelerator Laboratory, Menlo Park, CA 94025.
  • Liang M; Linac Coherent Light Source, Stanford Linear Accelerator Center National Accelerator Laboratory, Menlo Park, CA 94025.
  • Sierra RG; Linac Coherent Light Source, Stanford Linear Accelerator Center National Accelerator Laboratory, Menlo Park, CA 94025.
  • Lane TJ; Linac Coherent Light Source, Stanford Linear Accelerator Center National Accelerator Laboratory, Menlo Park, CA 94025.
  • Zhou L; Linac Coherent Light Source, Stanford Linear Accelerator Center National Accelerator Laboratory, Menlo Park, CA 94025.
  • Weierstall U; Linac Coherent Light Source, Stanford Linear Accelerator Center National Accelerator Laboratory, Menlo Park, CA 94025.
  • Zatsepin NA; Department of Physics, Beijing Normal University, Haidian, 100875 Beijing, People's Republic of China.
  • Ohki M; Department of Physics, Arizona State University, Tempe, AZ 85287.
  • Tame JRH; Department of Physics, Arizona State University, Tempe, AZ 85287.
  • Park SY; Department of Chemistry and Physics, Australian Research Council Centre of Excellence in Advanced Molecular Imaging, La Trobe Institute for Molecular Science, La Trobe University, Melbourne, VIC 3086, Australia.
  • Spence JCH; Drug Design Laboratory, Graduate School of Medical Life Science, Yokohama City University, 230-0045 Yokohama, Japan.
  • Zhang W; Drug Design Laboratory, Graduate School of Medical Life Science, Yokohama City University, 230-0045 Yokohama, Japan.
  • Schmidt M; Drug Design Laboratory, Graduate School of Medical Life Science, Yokohama City University, 230-0045 Yokohama, Japan.
  • Lee W; Department of Physics, Arizona State University, Tempe, AZ 85287.
  • Liu H; Department of Physics, Beijing Normal University, Haidian, 100875 Beijing, People's Republic of China.
Proc Natl Acad Sci U S A ; 118(13)2021 03 30.
Article en En | MEDLINE | ID: mdl-33753488
ABSTRACT
Chloride ion-pumping rhodopsin (ClR) in some marine bacteria utilizes light energy to actively transport Cl- into cells. How the ClR initiates the transport is elusive. Here, we show the dynamics of ion transport observed with time-resolved serial femtosecond (fs) crystallography using the Linac Coherent Light Source. X-ray pulses captured structural changes in ClR upon flash illumination with a 550 nm fs-pumping laser. High-resolution structures for five time points (dark to 100 ps after flashing) reveal complex and coordinated dynamics comprising retinal isomerization, water molecule rearrangement, and conformational changes of various residues. Combining data from time-resolved spectroscopy experiments and molecular dynamics simulations, this study reveals that the chloride ion close to the Schiff base undergoes a dissociation-diffusion process upon light-triggered retinal isomerization.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Cloruros / Canales de Cloruro / Rodopsinas Microbianas Idioma: En Revista: Proc Natl Acad Sci U S A Año: 2021 Tipo del documento: Article País de afiliación: Corea del Sur
Texto completo
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XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Cloruros / Canales de Cloruro / Rodopsinas Microbianas Idioma: En Revista: Proc Natl Acad Sci U S A Año: 2021 Tipo del documento: Article País de afiliación: Corea del Sur