Synthesis of silver nanoparticles using Ziziphus nummularia leaf extract and evaluation of their antimicrobial, antioxidant, cytotoxic and genotoxic potential (4-in-1 system).
Artif Cells Nanomed Biotechnol
; 49(1): 354-366, 2021 Dec.
Article
en En
| MEDLINE
| ID: mdl-33792441
ABSTRACT
This study reports the synthesis of silver nanoparticles (AgNPs) from silver nitrate by leaf extract of a medicinal plant Ziziphus nummularia. The leaf extract acts as a reducing and stabilizing agent for the formation of nanoparticles. The green synthesized AgNPs were characterized by ultraviolet-visible (UV-vis) spectroscopy, Fourier transform infrared (FITR) spectroscopy, Thermogravimetric analysis (TGA), X-ray diffraction (XRD), transmission electron microscopy (TEM) analysis and evaluated their antimicrobial, antioxidant, cytotoxic and genotoxic potential. The UV-Vis spectroscopy showed a characteristic absorption peak at 430 nm due to surface plasma resonance. TEM analysis showed that synthesized AgNPs were spherical and oval with an average size of 25.96 nm. AgNPs showed effective antimicrobial activity (lowest MIC-0.625 µg/mL against Escherichia coli), synergistic antimicrobial activity (lowest ΣFIC 0.09 with chlormaphenicol against Corynebacterium rubrum) and antibiofilm activity. AgNPs showed strong DPPH activity with IC50 - 520 µg/mL and ABTS activity IC50 - 55 µg/mL and reducing capacity assessment. In vitro cytotoxic effect was evaluated by MTT assay against HeLa cells, breast cells and fibroblast cells. Genotoxic effect was evaluated by comet assay. AgNPs displayed dose-dependent cytotoxic and genotoxic effect. Our findings indicated that synthesized AgNPs could be considered as multifunctional and have great potential for use in biomedical applications.HighlightsSilver nanoparticles were synthesized using leaf extract of Ziziphus nummulariaCharacterization was done by various spectral techniquesAntimicrobial efficacy was demonstrated against an array of bacteriaAgNPs exhibited significant cytotoxic effect against HeLa cell lineAgNPs showed cytotoxicity and genotoxicity in a dose-dependent manner.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Plata
/
Extractos Vegetales
/
Hojas de la Planta
/
Ziziphus
/
Nanopartículas del Metal
Límite:
Humans
Idioma:
En
Revista:
Artif Cells Nanomed Biotechnol
Año:
2021
Tipo del documento:
Article
País de afiliación:
India