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Arsenic accumulation and speciation in two cultivars of Pteris cretica L. and characterization of arsenate reductase PcACR2 and arsenite transporter PcACR3 genes in the hyperaccumulating cv. Albo-lineata.
Popov, Marek; Zemanová, Veronika; Sácký, Jan; Pavlík, Milan; Leonhardt, Tereza; Matousek, Tomás; Kana, Antonín; Pavlíková, Daniela; Kotrba, Pavel.
Afiliación
  • Popov M; Department of Biochemistry and Microbiology, University of Chemistry and Technology, Prague, Technická 3, 166 28 Prague, Czech Republic; Department of Botany and Plant Physiology, Czech University of Life Sciences Prague, Kamýcká 129, 16500 Prague, Czech Republic.
  • Zemanová V; Isotope Laboratory, Institute of Experimental Botany, The Czech Academy of Sciences, Vídenská 1083, 14220 Prague, Czech Republic; Department of Agro-Environmental Chemistry and Plant Nutrition, Czech University of Life Sciences Prague, Kamýcká 129, 16500 Prague, Czech Republic.
  • Sácký J; Department of Biochemistry and Microbiology, University of Chemistry and Technology, Prague, Technická 3, 166 28 Prague, Czech Republic.
  • Pavlík M; Isotope Laboratory, Institute of Experimental Botany, The Czech Academy of Sciences, Vídenská 1083, 14220 Prague, Czech Republic.
  • Leonhardt T; Department of Biochemistry and Microbiology, University of Chemistry and Technology, Prague, Technická 3, 166 28 Prague, Czech Republic.
  • Matousek T; Institute of Analytical Chemistry, The Czech Academy of Sciences, Veverí 97, 602 00 Brno, Czech Republic.
  • Kana A; Department of Analytical Chemistry, University of Chemistry and Technology, Prague, Technická 5, 166 28 Prague, Czech Republic.
  • Pavlíková D; Department of Agro-Environmental Chemistry and Plant Nutrition, Czech University of Life Sciences Prague, Kamýcká 129, 16500 Prague, Czech Republic.
  • Kotrba P; Department of Biochemistry and Microbiology, University of Chemistry and Technology, Prague, Technická 3, 166 28 Prague, Czech Republic. Electronic address: pavel.kotrba@vscht.cz.
Ecotoxicol Environ Saf ; 216: 112196, 2021 Apr 10.
Article en En | MEDLINE | ID: mdl-33848737
Pollution and poisoning with carcinogenic arsenic (As) is of major concern globally. Interestingly, there are ferns that can naturally tolerate remarkably high As concentrations in soils while hyperaccumulating this metalloid in their fronds. Besides Pteris vittata in which As-related traits and molecular determinants have been studied in detail, the As hyperaccumulation status has been attributed also to Pteris cretica. We thus inspected two P. cretica cultivars, Parkerii and Albo-lineata, for As hyperaccumulation traits. The cultivars were grown in soils supplemented with 20, 100, and 250 mg kg-1 of inorganic arsenate (iAsV). Unlike Parkerii, Albo-lineata was confirmed to be As tolerant and hyperaccumulating, with up to 1.3 and 6.4 g As kg-1 dry weight in roots and fronds, respectively, from soils amended with 250 mg iAsV kg-1. As speciation analyses rejected that organoarsenical species and binding with phytochelatins and other proteinaceous ligands would play any significant role in the biology of As in either cultivar. While in Parkerii, the dominating As species, particularly in roots, occurred as iAsV, in Albo-lineata the majority of the root and frond As was apparently converted to iAsIII. Parkerii markedly accumulated iAsIII in its fronds when grown on As spiked soils. Considering the roles iAsV reductase ACR2 and iAsIII transporter ACR3 may have in the handling of iAs, we isolated Albo-lineata PcACR2 and PcACR3 genes closely related to P. vittata PvACR2 and PvACR3. The gene expression analysis in Albo-lineata fronds revealed that the transcription of PcACR2 and PcACR3 was clearly As responsive (up to 6.5- and 45-times increase in transcript levels compared to control soil conditions, respectively). The tolerance and uptake assays in yeasts showed that PcACRs can complement corresponding As-sensitive mutations, indicating that PcACR2 and PcACR3 encode functional proteins that can perform, respectively, iAsV reduction and membrane iAsIII transport tasks in As-hyperaccumulating Albo-lineata.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Ecotoxicol Environ Saf Año: 2021 Tipo del documento: Article País de afiliación: República Checa

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Ecotoxicol Environ Saf Año: 2021 Tipo del documento: Article País de afiliación: República Checa
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