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Concerted evolution reveals co-adapted amino acid substitutions in Na+K+-ATPase of frogs that prey on toxic toads.
Mohammadi, Shabnam; Yang, Lu; Harpak, Arbel; Herrera-Álvarez, Santiago; Del Pilar Rodríguez-Ordoñez, María; Peng, Julie; Zhang, Karen; Storz, Jay F; Dobler, Susanne; Crawford, Andrew J; Andolfatto, Peter.
Afiliación
  • Mohammadi S; School of Biological Sciences, University of Nebraska, Lincoln, NE, USA.
  • Yang L; Department of Ecology and Evolutionary Biology, Princeton University, Princeton, NJ, USA.
  • Harpak A; Department of Biological Sciences, Columbia University, New York, NY, USA.
  • Herrera-Álvarez S; Department of Biological Sciences, Universidad de los Andes, Bogotá 111711, Colombia.
  • Del Pilar Rodríguez-Ordoñez M; Department of Biological Sciences, Universidad de los Andes, Bogotá 111711, Colombia.
  • Peng J; Lewis-Sigler Institute, Princeton University, Princeton, NJ, USA.
  • Zhang K; Department of Ecology and Evolutionary Biology, Princeton University, Princeton, NJ, USA.
  • Storz JF; School of Biological Sciences, University of Nebraska, Lincoln, NE, USA.
  • Dobler S; Molecular Evolutionary Biology, Zoological Institute, Universität Hamburg, Hamburg, Germany.
  • Crawford AJ; Department of Biological Sciences, Universidad de los Andes, Bogotá 111711, Colombia. Electronic address: andrew@dna.ac.
  • Andolfatto P; Department of Biological Sciences, Columbia University, New York, NY, USA. Electronic address: pa2543@columbia.edu.
Curr Biol ; 31(12): 2530-2538.e10, 2021 06 21.
Article en En | MEDLINE | ID: mdl-33887183
Although gene duplication is an important source of evolutionary innovation, the functional divergence of duplicates can be opposed by ongoing gene conversion between them. Here, we report on the evolution of a tandem duplication of Na+,K+-ATPase subunit α1 (ATP1A1) shared by frogs in the genus Leptodactylus, a group of species that feeds on toxic toads. One ATP1A1 paralog evolved resistance to toad toxins although the other retained ancestral susceptibility. Within species, frequent non-allelic gene conversion homogenized most of the sequence between the two copies but was counteracted by strong selection on 12 amino acid substitutions that distinguish the two paralogs. Protein-engineering experiments show that two of these substitutions substantially increase toxin resistance, whereas the additional 10 mitigate their deleterious effects on ATPase activity. Our results reveal how examination of neo-functionalized gene duplicate evolution can help pinpoint key functional substitutions and interactions with the genetic backgrounds on which they arise.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Anuros / Conducta Predatoria / Adaptación Fisiológica / ATPasa Intercambiadora de Sodio-Potasio / Evolución Molecular / Sustitución de Aminoácidos / Ingestión de Alimentos Límite: Animals Idioma: En Revista: Curr Biol Asunto de la revista: BIOLOGIA Año: 2021 Tipo del documento: Article País de afiliación: Estados Unidos

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Anuros / Conducta Predatoria / Adaptación Fisiológica / ATPasa Intercambiadora de Sodio-Potasio / Evolución Molecular / Sustitución de Aminoácidos / Ingestión de Alimentos Límite: Animals Idioma: En Revista: Curr Biol Asunto de la revista: BIOLOGIA Año: 2021 Tipo del documento: Article País de afiliación: Estados Unidos
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