A Workflow for High-pressure Freezing and Freeze Substitution of the <i>Caenorhabditis elegans</i> Embryo for Ultrastructural Analysis by Conventional and Volume Electron Microscopy.

Rahman, Mohammad M; Chang, Irene Y; Cohen-Fix, Orna; Narayan, Kedar

A Workflow for High-pressure Freezing and Freeze Substitution of the Caenorhabditis elegans Embryo for Ultrastructural Analysis by Conventional and Volume Electron Microscopy.

Rahman, Mohammad M; Chang, Irene Y; Cohen-Fix, Orna; Narayan, Kedar.

Afiliación

Rahman MM; The Laboratory of Biochemistry and Genetics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD, USA.
Chang IY; Cancer Research Technology Program, Frederick National Laboratory for Cancer Research, Frederick, MD, USA.
Cohen-Fix O; Center for Molecular Microscopy, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Frederick, MD, USA.
Narayan K; The Laboratory of Biochemistry and Genetics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD, USA.

Bio Protoc ; 11(7): e3981, 2021 Apr 05.

Article en En | MEDLINE | ID: mdl-33889675

Palabras clave

C. elegans; FIB-SEM; Freeze substitution; High-pressure freezing; Volume electron microscopy; vEM

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Bio Protoc Año: 2021 Tipo del documento: Article País de afiliación: Estados Unidos

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