Protocol for behavioral tests using chemogenetically manipulated mice.
STAR Protoc
; 2(2): 100418, 2021 06 18.
Article
en En
| MEDLINE
| ID: mdl-33899009
ABSTRACT
Behavioral analyses using mice chemogenetically manipulated by designer receptors exclusively activated by designer drugs (DREADDs) are powerful tools to elucidate neural functions. Here, we describe the detailed protocols for stereotaxic surgery, adeno-associated virus (AAV)-mediated introduction to Gq-DREADDs in mice, and for behavioral testing and analyses related to anxiety, risk assessment, and burying behaviors. A series of these tests are useful in evaluating animal anxiety and their defensive response patterns to potential threats. For complete details on the use and execution of this protocol, please refer to Horii-Hayashi et al. (2021).
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Receptores de Droga
/
Conducta Animal
/
Ratones Transgénicos
/
Drogas de Diseño
/
Escala de Evaluación de la Conducta
Tipo de estudio:
Risk_factors_studies
Límite:
Animals
Idioma:
En
Revista:
STAR Protoc
Año:
2021
Tipo del documento:
Article
País de afiliación:
Japón