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Development of Anti-human T Cell Immunoreceptor with Ig and ITIM Domains (TIGIT) Monoclonal Antibodies for Flow Cytometry.
Takei, Junko; Asano, Teizo; Nanamiya, Ren; Nakamura, Takuro; Yanaka, Miyuki; Hosono, Hideki; Tanaka, Tomohiro; Sano, Masato; Kaneko, Mika K; Harada, Hiroyuki; Kato, Yukinari.
Afiliación
  • Takei J; Department of Antibody Drug Development, Tohoku University Graduate School of Medicine, Sendai, Japan.
  • Asano T; Department of Oral and Maxillofacial Surgery, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Bunkyo-ku, Japan.
  • Nanamiya R; Department of Antibody Drug Development, Tohoku University Graduate School of Medicine, Sendai, Japan.
  • Nakamura T; Department of Antibody Drug Development, Tohoku University Graduate School of Medicine, Sendai, Japan.
  • Yanaka M; Department of Antibody Drug Development, Tohoku University Graduate School of Medicine, Sendai, Japan.
  • Hosono H; Department of Antibody Drug Development, Tohoku University Graduate School of Medicine, Sendai, Japan.
  • Tanaka T; Department of Antibody Drug Development, Tohoku University Graduate School of Medicine, Sendai, Japan.
  • Sano M; Department of Antibody Drug Development, Tohoku University Graduate School of Medicine, Sendai, Japan.
  • Kaneko MK; Department of Antibody Drug Development, Tohoku University Graduate School of Medicine, Sendai, Japan.
  • Harada H; Department of Antibody Drug Development, Tohoku University Graduate School of Medicine, Sendai, Japan.
  • Kato Y; Department of Oral and Maxillofacial Surgery, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Bunkyo-ku, Japan.
Monoclon Antib Immunodiagn Immunother ; 40(2): 71-75, 2021 Apr.
Article en En | MEDLINE | ID: mdl-33900817
Immune checkpoint inhibitors targeting programmed cell death-ligand 1 (PD-L1), programmed cell death-1 (PD-1), and cytotoxic T lymphocyte-associated antigen 4 (CTLA-4) recently made a significant survival rate improvement in cancer treatment. T cell immunoreceptor with Ig and ITIM domains (TIGIT) is expressed in T and NK cells related to their activities. It has a single extracellular immunoglobulin domain, a type 1 transmembrane domain, and a single intracellular ITIM. TIGIT binds with poliovirus receptor (PVR) or PVR2, resulting in suppressing T and NK cell activities. Some studies showed that the combined use of a TIGIT inhibitor with another immune checkpoint inhibitor enhanced antitumor activities more strongly than their single use. Therefore, TIGIT should be a new target for immunotherapy. In this study, we developed new anti-human TIGIT (hTIGIT) monoclonal antibodies (mAbs) using the Cell-Based Immunization and Screening (CBIS) method. Mice were immunized with hTIGIT-overexpressed Chinese hamster ovary (CHO)-K1 cells (CHO/hTIGIT), and hybridomas were screened by flow cytometry. One of the mAbs, TgMab-2 (IgG1, kappa), specifically and sensitively detects hTIGIT in CHO/hTIGIT and NK cells. The dissociation constants (KD) of TgMab-2 for CHO/hTIGIT cells were determined to be 3.5 × 10-9 M. These results suggest that TgMab-2, which was developed by CBIS method, is useful for analyzing the function of hTIGIT by flow cytometry.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Contexto en salud: 6_ODS3_enfermedades_notrasmisibles Problema de salud: 6_ovary_cancer Asunto principal: Receptores Inmunológicos / Inhibidores de Puntos de Control Inmunológico / Anticuerpos Monoclonales / Neoplasias Límite: Animals / Humans Idioma: En Revista: Monoclon Antib Immunodiagn Immunother Año: 2021 Tipo del documento: Article País de afiliación: Japón

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Contexto en salud: 6_ODS3_enfermedades_notrasmisibles Problema de salud: 6_ovary_cancer Asunto principal: Receptores Inmunológicos / Inhibidores de Puntos de Control Inmunológico / Anticuerpos Monoclonales / Neoplasias Límite: Animals / Humans Idioma: En Revista: Monoclon Antib Immunodiagn Immunother Año: 2021 Tipo del documento: Article País de afiliación: Japón
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