Circulating microRNA135a3p in serum extracellular vesicles as a potential biological marker of nonalcoholic fatty liver disease.
Mol Med Rep
; 24(1)2021 07.
Article
en En
| MEDLINE
| ID: mdl-33955511
ABSTRACT
Nonalcoholic fatty liver disease (NAFLD) is a widespread threat to human health. However, the present screening methods for NAFLD are timeconsuming or invasive. The present study aimed to assess the potential of microRNAs (miRNAs/miRs) in serum extracellular vesicles (EVs) as a biomarker of NAFLD. C57BL/6J mice were fed either a 12week highfat diet (HFD) or standard chow to establish NAFLD and control groups, respectively. Serum samples were obtained from the mouse model of NAFLD, as well as 50 patients with NAFLD and 50 healthy individuals, and EVs were extracted and verified. Using reverse transcriptionquantitative PCR, the mRNA expression level of selected miRNAs in the serum and EVs was analyzed. In order to determine the diagnostic value, receiver operating characteristic (ROC) curves were used. The mice treated with HFD showed notable hepatic steatosis and higher concentrations of serum alanine aminotransferase (ALT). There was also a significant decrease in the expression levels of miR135a3p, miR129b5p and miR5043p, and an increase in miR1225p expression levels in circulating EVs in mice treated with HFD and patients with NAFLD. There were also similar miR135a3p and miR1225p expression patterns in the serum. ROC analysis demonstrated that miR135a3p in circulating EVs was highly accurate in diagnosing NAFLD, with the area under the curve value being 0.849 (95% CI, 0.7770.921; P<0.0001). Bioinformatics analysis indicated that dysregulated miR135a3p was associated with 'plateletderived growth factor receptor signaling pathway' and 'AMPactivated protein kinase signaling pathway'. In summary, circulating miR135a3p in EVs may serve as a potential noninvasive biomarker to diagnose NAFLD. This miRNA was a more sensitive and specific biological marker for NAFLD compared with ALT.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
MicroARNs
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Enfermedad del Hígado Graso no Alcohólico
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Vesículas Extracelulares
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MicroARN Circulante
Tipo de estudio:
Prognostic_studies
Límite:
Adult
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Animals
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Female
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Humans
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Male
Idioma:
En
Revista:
Mol Med Rep
Año:
2021
Tipo del documento:
Article