A robust method of nuclei isolation for single-cell RNA sequencing of solid tissues from the plant genus Populus.

Conde, Daniel; Triozzi, Paolo M; Balmant, Kelly M; Doty, Andria L; Miranda, Mariza; Boullosa, Anthony; Schmidt, Henry W; Pereira, Wendell J; Dervinis, Christopher; Kirst, Matias

Conde, Daniel; Triozzi, Paolo M; Balmant, Kelly M; Doty, Andria L; Miranda, Mariza; Boullosa, Anthony; Schmidt, Henry W; Pereira, Wendell J; Dervinis, Christopher; Kirst, Matias.

Afiliación

Conde D; School of Forest, Fisheries and Geomatic Sciences, University of Florida, Gainesville, Florida, United States of America.
Triozzi PM; School of Forest, Fisheries and Geomatic Sciences, University of Florida, Gainesville, Florida, United States of America.
Balmant KM; School of Forest, Fisheries and Geomatic Sciences, University of Florida, Gainesville, Florida, United States of America.
Doty AL; Interdisciplinary Center for Biotechnology, Flow Cytometry & Imaging Core, University of Florida, Gainesville, Florida, United States of America.
Miranda M; Interdisciplinary Center for Biotechnology, Flow Cytometry & Imaging Core, University of Florida, Gainesville, Florida, United States of America.
Boullosa A; Interdisciplinary Center for Biotechnology, Flow Cytometry & Imaging Core, University of Florida, Gainesville, Florida, United States of America.
Schmidt HW; School of Forest, Fisheries and Geomatic Sciences, University of Florida, Gainesville, Florida, United States of America.
Pereira WJ; School of Forest, Fisheries and Geomatic Sciences, University of Florida, Gainesville, Florida, United States of America.
Dervinis C; School of Forest, Fisheries and Geomatic Sciences, University of Florida, Gainesville, Florida, United States of America.
Kirst M; School of Forest, Fisheries and Geomatic Sciences, University of Florida, Gainesville, Florida, United States of America.

PLoS One ; 16(5): e0251149, 2021.

Article en En | MEDLINE | ID: mdl-33974645

Asunto(s)

Fraccionamiento Celular/métodos; Populus/genética; ARN de Planta; Análisis de Secuencia de ARN; Perfilación de la Expresión Génica/métodos; Protoplastos; Reproducibilidad de los Resultados; Análisis de la Célula Individual

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Fraccionamiento Celular / Análisis de Secuencia de ARN / ARN de Planta / Populus Tipo de estudio: Prognostic_studies Idioma: En Revista: PLoS One Asunto de la revista: CIENCIA / MEDICINA Año: 2021 Tipo del documento: Article País de afiliación: Estados Unidos

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