The structure, conformation, and hypoglycemic activity of a novel heteropolysaccharide from the blackberry fruit.
Food Funct
; 12(12): 5451-5464, 2021 Jun 21.
Article
en En
| MEDLINE
| ID: mdl-33997873
ABSTRACT
A novel heteropolysaccharide fraction (BBP-24-3) with a relative molecular weight of 145.1 kDa was isolated from blackberry fruits. The BBP-24-3 was mainly composed of arabinose, glucose, and galacturonic acid with a ratio of 5.30 3.60 91.10 mol%. Structural analysis showed that BBP-24-3 possessed a 1,6-linked ß-d-Glcp, 1, 2, 3, 5 linked α-l-Araf, and 1, 4 linked α-d-GalpA backbone with branches substituted at the C-2 and C-5 positions of arabinose units, which included 1, 2, 3, 4 linked ß-d-Glcp and T-linked ß-d-GalpA. The conformation analysis indicated that BBP-24-3 exhibited a solid spherical structure with a uniform distribution in 0.1 M NaCl solution. The BBP-24-3 exhibited excellent α-glucosidase inhibitory activity with an IC50 value of 3.70 mg mL-1, which was due to the structural change, including α-helix and random coil of α-glucosidase caused by BBP-24-3. The current work suggests the potential utilization of BBP-24-3 as an α-glucosidase inhibitor in healthy food for reducing the postprandial blood glucose level.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Polisacáridos
/
Rubus
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Frutas
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Hipoglucemiantes
Idioma:
En
Revista:
Food Funct
Año:
2021
Tipo del documento:
Article