Effects of external low intensity focused ultrasound on inflammatory markers in neuropathic pain.
Neurosci Lett
; 757: 135977, 2021 07 13.
Article
en En
| MEDLINE
| ID: mdl-34023413
ABSTRACT
BACKGROUND:
Changes in inflammatory cytokine levels contribute to the induction and maintenance of neuropathic pain. We have shown that external low intensity focused ultrasound (liFUS) reduces allodynia in a common peroneal nerve injury (CPNI). Here, we investigate an underlying mechanism of action for this treatment and measure the effect of liFUS on inflammatory markers.METHODS:
Male rats were divided into four groups CPNI/liFUS, CPNI/shamliFUS, shamCPNI/liFUS, and shamCPNI/shamliFUS. Mechanical nociceptive thresholds were measured using Von Frey filaments (VFF) to confirm the absence/presence of allodynia at baseline, after CPNI, and after liFUS. Commercial microarray and ELISA assays were used to assess cytokine expression in the treated L5 dorsal root ganglion (DRG) and dorsal horn (DH) tissue 24 and 72â¯h after liFUS.RESULTS:
VFF thresholds were significantly reduced following CPNI in both groups that received the injury (pâ¯<â¯0.001). After liFUS, only the CPNI/liFUS cohort showed a significant increase in mechanical thresholds (pâ¯<â¯0.001). CPNI significantly increased TNFa, IL6, CNTF, IL1b (pâ¯<â¯0.05 for all) levels in the DRG and DH, compared to baseline, consistent with previous work in sciatic nerve injury. LiFUS in CPNI rats resulted in a decrease in these cytokines in DRG 72â¯h post-therapy (TNFa, IL6, CNTF and IL1b, pâ¯<â¯0.001). In the DH, IL1b, CNTF, and TNFa (pâ¯<â¯0.05 for all) decreased 72â¯h after liFUS.CONCLUSION:
We have demonstrated that liFUS modifies inflammatory cytokines in both DRG and DH in CPNI rats. These data provide evidence that liFUS, reverses the allodynic phenotype, in part, by altering inflammatory cytokine pathways.Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Terapia por Ultrasonido
/
Traumatismos de los Nervios Periféricos
/
Hiperalgesia
/
Neuralgia
Tipo de estudio:
Diagnostic_studies
/
Prognostic_studies
Límite:
Animals
/
Humans
/
Male
Idioma:
En
Revista:
Neurosci Lett
Año:
2021
Tipo del documento:
Article
País de afiliación:
Estados Unidos