Establishment of a C57BL/6 Mandibular Critical-Size Bone Defect Model.
J Craniofac Surg
; 32(7): 2562-2565, 2021 Oct 01.
Article
en En
| MEDLINE
| ID: mdl-34054086
ABSTRACT
PURPOSE:
The goal of this study was to identify bone defects of critical size in C57BL/6 mouse mandibles. MATERIALS ANDMETHODS:
Twenty-four male mice were included in this study. All mice underwent surgeries on their left mandibles. Mandibular defects of 1.0âmm (nâ=â8), 1.6âmm (nâ=â8), and 2.3âmm (nâ=â8) were created. For the investigation of bone healing after an 8-week period, micro-computed tomography scans and histomorphology were performed.RESULTS:
Mandibular bone nonunions were seen 0/8 in the 1.0-mm group, 6/8 in the 1.6-mm group, and 8/8 in the 2.3-mm group. The outcome of micro-computed tomography showed that, after 8âweeks, the bone mineral density and the bone volume to total volume ratio were significantly different among the 3 groups. The defect gaps in the nonunion 1.6- and 2.3-mm groups were filled with connective tissue, and no obvious bone formation was found. Additionally, in quantitative analysis, according to the new bone fill calculations, the percentages were 91.85%â±â8.03% in the 1.0-mm group, 59.84%â±â20.60% in the 1.6-mm group, and 15.36%â±â8.28% in the 2.3-mm group, which indicated statistically significantly lower defect healing in the 2.3-mm group.CONCLUSIONS:
The creation of 2.3-mm mandibular defects produces osseous nonunion in C57BL/6 mice.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Osteogénesis
/
Mandíbula
Límite:
Animals
Idioma:
En
Revista:
J Craniofac Surg
Asunto de la revista:
ODONTOLOGIA
Año:
2021
Tipo del documento:
Article
País de afiliación:
China