Single-cell analyses reveal suppressive tumor microenvironment of human colorectal cancer.
Clin Transl Med
; 11(6): e422, 2021 06.
Article
en En
| MEDLINE
| ID: mdl-34185431
ABSTRACT
Profiling heterologous cell types within tumors is essential to decipher tumor microenvironment that shapes tumor progress and determines the outcome of therapeutic response. Here, we comprehensively characterized transcriptomes of 34,037 single cells obtained from 12 treatment-naïve patients with colorectal cancer. Our comprehensive evaluation revealed attenuated B-cell antigen presentation, distinct regulatory T-cell clusters with different origin and novel polyfunctional tumor associated macrophages associated with CRC. Moreover, we identified expanded XCL1+ T-cell clusters associated with tumor mutational burden high status. We further explored the underlying molecular mechanisms by profiling epigenetic landscape and inferring transcription factor motifs using single-cell ATAC-seq. Our dataset and analysis approaches herein provide a rich resource for further study of the impact of immune cells and translational research for human colorectal cancer.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Neoplasias Colorrectales
/
Biomarcadores de Tumor
/
Regulación Neoplásica de la Expresión Génica
/
Análisis de la Célula Individual
/
Microambiente Tumoral
/
Transcriptoma
Tipo de estudio:
Prognostic_studies
Límite:
Humans
Idioma:
En
Revista:
Clin Transl Med
Año:
2021
Tipo del documento:
Article
País de afiliación:
China