Determination of cortisol in hair using liquid chromatography-tandem mass spectrometry: a short review.
Bioanalysis
; 13(14): 1145-1155, 2021 Jul.
Article
en En
| MEDLINE
| ID: mdl-34187201
ABSTRACT
Cortisol is considered a particularly relevant biomarker in the context of stress evaluation. This study aims to review of the available literature on the determination of cortisol in hair using LC-MS/MS. Currently, there is no standardized procedure for the measurement of cortisol concentrations in hair, and different sample preparation, chromatographic separation and mass spectrometric detection conditions were described. Simple methanolic extraction, reversed-phase separation and MRM detection in negative ion mode are the most common employed analytical approaches. Reported assays presented acceptable sensitivity for clinical purposes. The increasing use of mass spectrometry in clinical laboratories may contribute to the establishment of LC-MS/MS as the method of choice for the determination of cortisol concentrations in hair.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Hidrocortisona
/
Cromatografía Liquida
/
Espectrometría de Masas en Tándem
/
Cabello
Tipo de estudio:
Systematic_reviews
Límite:
Humans
Idioma:
En
Revista:
Bioanalysis
Año:
2021
Tipo del documento:
Article
País de afiliación:
Brasil