Determination of Azide Ions in Blood by Pentafluorobenzyl Derivation Followed by GC-MS.
Fa Yi Xue Za Zhi
; 37(3): 378-381, 2021 Jun.
Article
en En, Zh
| MEDLINE
| ID: mdl-34379908
ABSTRACT
ABSTRACT Objective To establish a method for determination of the azide ions in blood by gas chromatography-mass spectrometry ï¼GC-MSï¼ following pentafluorobenzyl derivatization. Methods A blood sample of 0.2 mL was placed into a 10 mL glass test tube, and the internal standard sodium cyanide, derivatization reagent pentafluorobenzyl bromide and catalyst tetradecyl benzyl dimethyl ammonium chloride were added in turn. After vortex mixing, the mixture was heated with low-power microwave for 3 min. After centrifugation, the organic phase was taken for GC-MS analysis. Results The azide ions in blood had a good linear relationship in the mass concentration range of 0.5 to 20 µg/mL. The lowest detection limit was 0.25 µg/mL and the relative recovery was 91.36%-94.58%. The method was successfully applied to a case of death from sodium azide poisoning. The mass concentration of azide ions in the blood of the dead was 11.11 µg/mL. Conclusion The method developed in this paper has strong specificity and is easy to operate, which is suitable for the rapid detection of azide ions in blood.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Azidas
Límite:
Humans
Idioma:
En
/
Zh
Revista:
Fa Yi Xue Za Zhi
Asunto de la revista:
JURISPRUDENCIA
Año:
2021
Tipo del documento:
Article
País de afiliación:
China