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Diversity of Non-O157 Shiga Toxin-Producing Escherichia coli Isolated from Cattle from Central and Southern Chile.
Díaz, Leonela; Gutierrez, Sebastian; Moreno-Switt, Andrea I; Hervé, Luis Pablo; Hamilton-West, Christopher; Padola, Nora Lía; Navarrete, Paola; Reyes-Jara, Angélica; Meng, Jianghong; González-Escalona, Narjol; Toro, Magaly.
Afiliación
  • Díaz L; Instituto de Nutrición y Tecnología de los Alimentos (INTA), Universidad de Chile, Macul 7830490, Santiago, Chile.
  • Gutierrez S; Instituto de Nutrición y Tecnología de los Alimentos (INTA), Universidad de Chile, Macul 7830490, Santiago, Chile.
  • Moreno-Switt AI; Escuela de Medicina Veterinaria, Facultad de Agronomía e Ingeniería Forestal, Facultad de Ciencias Biológicas, Facultad de Medicina, Pontificia Universidad Católica de Chile, Macul 7820244, Santiago, Chile.
  • Hervé LP; ANID-Millennium Initiative for Collaborative Research on Bacterial Resistance (MICROB-R), Avenida Las Condes 12461, Lo Barnechea 7690000, Santiago, Chile.
  • Hamilton-West C; Facultad de Ciencias Veterinarias y Pecuarias, Universidad de Chile, La Pintana 8820808, Santiago, Chile.
  • Padola NL; Biomedical Science Department, Ross University School of Veterinary Medicine, Basseterre KN269, Saint Kitts and Nevis.
  • Navarrete P; Facultad de Ciencias Veterinarias y Pecuarias, Universidad de Chile, La Pintana 8820808, Santiago, Chile.
  • Reyes-Jara A; Centro de Investigación Veterinaria Tandil, CONICET-CIC, Facultad de Ciencias Veterinarias, UNCPBA, Tandil 7000, Buenos Aires, Argentina.
  • Meng J; Instituto de Nutrición y Tecnología de los Alimentos (INTA), Universidad de Chile, Macul 7830490, Santiago, Chile.
  • González-Escalona N; ANID-Millennium Science Initiative Program-Millennium Nucleus in the Biology of the Intestinal Microbiota, Macul 7830490, Santiago, Chile.
  • Toro M; Instituto de Nutrición y Tecnología de los Alimentos (INTA), Universidad de Chile, Macul 7830490, Santiago, Chile.
Animals (Basel) ; 11(8)2021 Aug 13.
Article en En | MEDLINE | ID: mdl-34438845
Cattle are the main reservoir of Shiga toxin-producing Escherichia coli (STEC), one of the world's most important foodborne pathogens. The pathogen causes severe human diseases and outbreaks. This study aimed to identify and characterize non-O157 STEC isolated from cattle feces from central and southern Chile. We analyzed 446 cattle fecal samples and isolated non-O157 STEC from 12.6% (56/446); a total of 93 different isolates were recovered. Most isolates displayed ß-glucuronidase activity (96.8%; 90/93) and fermented sorbitol (86.0%; 80/93), whereas only 39.8% (37/93) were resistant to tellurite. A subgroup of 30 representative non-O157 STEC isolates was selected for whole-genome sequencing and bioinformatics analysis. In silico analysis showed that they grouped into 16 different sequence types and 17 serotypes; the serotypes most frequently identified were O116:H21 and O168:H8 (13% each). A single isolate of serotype O26:H11 was recovered. One isolate was resistant to tetracycline and carried resistance genes tet(A) and tet(R); no other isolate displayed antimicrobial resistance or carried antimicrobial resistance genes. The intimin gene (eae) was identified in 13.3% (4/30) of the genomes and 90% (27/30) carried the stx2 gene. A phylogenetic reconstruction demonstrated that the isolates clustered based on serotypes, independent of geographical origin. These results indicate that cattle in Chile carry a wide diversity of STEC potentially pathogenic for humans based on the presence of critical virulence genes.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Contexto en salud: 3_ND Problema de salud: 3_zoonosis País/Región como asunto: America do sul / Chile Idioma: En Revista: Animals (Basel) Año: 2021 Tipo del documento: Article País de afiliación: Chile

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Contexto en salud: 3_ND Problema de salud: 3_zoonosis País/Región como asunto: America do sul / Chile Idioma: En Revista: Animals (Basel) Año: 2021 Tipo del documento: Article País de afiliación: Chile
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