Increasing transforming growth factor-beta concentrations with age decrease apelin in the rat rotator cuff.
J Orthop Surg Res
; 16(1): 539, 2021 Aug 31.
Article
en En
| MEDLINE
| ID: mdl-34465345
ABSTRACT
BACKGROUND:
The rotator cuff undergoes natural degeneration with age, leading to age-related rotator cuff tear; however, the precise mechanism remains unclear. Transforming growth factor-beta (TGF-ß) concentrations rise with age and TGF-ß contributes to the pathophysiology of skeletal muscle. TGF-ß has also been shown to suppress expression of the myokine, apelin, in skin fibroblasts. We hypothesized that TGF-ß expression in the rotator cuff changes with age and regulates apelin expression, thereby contributing to rotator cuff degeneration.METHODS:
We used quantitative reverse-transcription polymerase chain reaction (Q-RT-PCR) to measure the expression of apelin and tendon-related genes (Tnmd, Col1a1, and Col3a1) in the rotator cuff of young (12 weeks), adult (24 weeks), and old (48 weeks) rats. Using Q-RT-PCR and enzyme-linked immunosorbent assay, we also measured Tgfb mRNA and TGF-ß protein levels, respectively. Furthermore, we used Q-RT-PCR to measure apelin mRNA levels in rotator cuff-derived cells after treatment with 0 (control) and 10 ng/mL recombinant TGF-ß.RESULTS:
Apelin mRNA levels were significantly lower in old compared to young and adult rats. Similarly, tendon-related genes, Tnmd, Col1a1, and Col3a1, were significantly lower in adult and old rats than young rats. In contrast, Tgfb mRNA and TGF-ß protein were significantly higher in old compared to young rats. Stimulation with exogenous TGF-ß significantly decreased Apelin mRNA expression compared to control.CONCLUSIONS:
TGF-ß regulates apelin expression in the rotator cuff and may play a key role in the degenerative pathology of the rotator cuff with age.Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Manguito de los Rotadores
/
Lesiones del Manguito de los Rotadores
Límite:
Animals
Idioma:
En
Revista:
J Orthop Surg Res
Año:
2021
Tipo del documento:
Article
País de afiliación:
Japón