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A systematic investigation of the effect of sample solvent on peak shape in nano- and microflow hydrophilic interaction liquid chromatography columns.
Li, Haibin; Liu, Chusheng; Zhao, Li; Xu, Dongsheng; Zhang, Tingting; Wang, Qiqin; Cabooter, Deirdre; Jiang, Zhengjin.
Afiliación
  • Li H; Institute of Pharmaceutical Analysis, College of Pharmacy, Jinan University, Guangzhou 510632, China; International Cooperative Laboratory of Traditional Chinese Medicine Modernization and Innovative Drug Development of Chinese Ministry of Education (MOE), College of Pharmacy, Jinan University, Guan
  • Liu C; NMPA Key Laboratory for Monitoring and Evaluation of Cosmetics, Shenzhen Institute for Drug Control, Shenzhen 518057, China.
  • Zhao L; Institute of Pharmaceutical Analysis, College of Pharmacy, Jinan University, Guangzhou 510632, China; International Cooperative Laboratory of Traditional Chinese Medicine Modernization and Innovative Drug Development of Chinese Ministry of Education (MOE), College of Pharmacy, Jinan University, Guan
  • Xu D; Institute of Pharmaceutical Analysis, College of Pharmacy, Jinan University, Guangzhou 510632, China; International Cooperative Laboratory of Traditional Chinese Medicine Modernization and Innovative Drug Development of Chinese Ministry of Education (MOE), College of Pharmacy, Jinan University, Guan
  • Zhang T; Institute of Pharmaceutical Analysis, College of Pharmacy, Jinan University, Guangzhou 510632, China; International Cooperative Laboratory of Traditional Chinese Medicine Modernization and Innovative Drug Development of Chinese Ministry of Education (MOE), College of Pharmacy, Jinan University, Guan
  • Wang Q; Institute of Pharmaceutical Analysis, College of Pharmacy, Jinan University, Guangzhou 510632, China; International Cooperative Laboratory of Traditional Chinese Medicine Modernization and Innovative Drug Development of Chinese Ministry of Education (MOE), College of Pharmacy, Jinan University, Guan
  • Cabooter D; Department for Pharmaceutical and Pharmacological Sciences, KU Leuven, Pharmaceutical Analysis, Herestraat 49, Leuven 3000, Belgium. Electronic address: deirdre.cabooter@kuleuven.be.
  • Jiang Z; Institute of Pharmaceutical Analysis, College of Pharmacy, Jinan University, Guangzhou 510632, China; International Cooperative Laboratory of Traditional Chinese Medicine Modernization and Innovative Drug Development of Chinese Ministry of Education (MOE), College of Pharmacy, Jinan University, Guan
J Chromatogr A ; 1655: 462498, 2021 Oct 11.
Article en En | MEDLINE | ID: mdl-34496327
A frequently encountered problem in the practical application of nano- and microflow hydrophilic interaction chromatography (HILIC) columns is the distortion of peak shapes arising from a mismatch between the sample solvent and the mobile phase. An unmatched or improperly matched sample solvent can distort the peak shape of analytes and influence their retention times, thereby affecting the quality of the resulting chromatogram. In this work, the effect of sample solvent composition (mixtures of acetonitrile, water, methanol and isopropanol in different ratios) and injection volume (20-100 nL) was systematically investigated using a selection of neutral and charged compounds on a series of zwitterionic and charged small I.D. (0.1-0.3 mm) HILIC columns. For retained compounds, pure ACN was demonstrated to be the best sample solvent to obtain narrow peaks, while for compounds that eluted very close to the solvent peak, the peak shape was distorted when the sample solvent consisted of pure ACN. A highly aqueous sample solvent, which interferes with the partitioning of polar analytes into the stationary phase, was demonstrated to be detrimental for the peak shape of retained neutral compounds, while for unretained compounds that do not or hardly interact with the stationary phase, a high amount of water in the sample solvent was not problematic. For charged compounds, water in the sample solvent favored the electrostatic attraction with the stationary phase. Therefore, the retention time of charged analytes was shown to increase with increasing water content in the sample solvent. Even when a large amount of water was present in the sample solvent, the peak shapes of these compounds were still acceptable. For highly polar compounds with a limited solubility in aqueous sample solvents, it was found that a mixture of ACN and MeOH or IPA is a good alternative.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Agua / Metanol Idioma: En Revista: J Chromatogr A Año: 2021 Tipo del documento: Article

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Agua / Metanol Idioma: En Revista: J Chromatogr A Año: 2021 Tipo del documento: Article
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