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Low-intensity pulsed ultrasound promotes angiogenesis via the AKT pathway and DNA methylation in human umbilical vein endothelial cells.
Li, JiaYi; Guo, WeiMing; Yu, Fei; Liu, Lu; Wang, XiaoTing; Li, LvYuan; Fang, Bing; Xia, Lunguo.
Afiliación
  • Li J; School of Medicine, Shanghai Jiao Tong University, 227 South Chongqing Road, Shanghai 200025, China.
  • Guo W; School of Medicine, Shanghai Jiao Tong University, 227 South Chongqing Road, Shanghai 200025, China.
  • Yu F; School of Medicine, Shanghai Jiao Tong University, 227 South Chongqing Road, Shanghai 200025, China.
  • Liu L; Department of Orthodontics, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, 500 Qu Xi Road, Shanghai 200011, China.
  • Wang X; Department of Orthodontics, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, 500 Qu Xi Road, Shanghai 200011, China.
  • Li L; Department of Orthodontics, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, 500 Qu Xi Road, Shanghai 200011, China.
  • Fang B; Department of Orthodontics, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, 500 Qu Xi Road, Shanghai 200011, China. Electronic address: fangbing@sjtu.edu.cn.
  • Xia L; Department of Orthodontics, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, 500 Qu Xi Road, Shanghai 200011, China. Electronic address: xialunguo@hotmail.com.
Ultrasonics ; 118: 106561, 2022 Jan.
Article en En | MEDLINE | ID: mdl-34500338
Angiogenesis involves the activation of endothelial cells (ECs). Low-intensity pulsed ultrasound (LIPUS), which delivers ultrasound waves at a low intensity, can induce the angiogenic potential of ECs. However, the underlying cellular mechanisms remain to be elucidated. In this study, the LIPUS parameters were 1.5 MHz pulsed frequency, 200 us pulse duration, 1.0 kHz repetition rate, and 30 mW/cm2 energy intensity. First, we evaluated the effects of LIPUS on the proliferation and angiogenic differentiation of the EC line EA.hy926. The results showed that LIPUS could induce cell proliferation, promote migration, and increase mRNA level inKDR and CD144.Also, the mRNA level and secretion of VEGF were enhanced. We then investigated the role of the AKT signaling pathway in this process. We observed that the expression of p-AKT was upregulated which means that the AKT signaling pathway could be activated by LIPUS, while inhibitor LY294002 of the AKT signaling pathway effectively blocked LIPUS-induced angiogenesis. Finally,we applied confocal Raman microscopy to track biomolecular changes in cells after LIPUS treatment. Spectral analysis showed DNA methylation changes. An Infinium Methylation assay suggested that399 sites were significantly different. After KEGG enrichment analysis, we found seven genes (IRS1, GNG7, COL4A1, FOXO3, COL4A2, CDK4 and EGF) which were closely related to AKT signaling pathway. We verified that AKT signaling pathway inhibition partially blocked LIPUS-induced DNA methylation changes. Ourstudy demonstrated that LIPUS couldpromote the proliferation and angiogenic differentiation of ECs via the AKT signaling pathway. LIPUS could also alter DNA methylation of ECs via the activation of AKT signal.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neovascularización Fisiológica / Metilación de ADN / Proteínas Proto-Oncogénicas c-akt / Células Endoteliales de la Vena Umbilical Humana / Ondas Ultrasónicas Límite: Humans Idioma: En Revista: Ultrasonics Año: 2022 Tipo del documento: Article País de afiliación: China

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neovascularización Fisiológica / Metilación de ADN / Proteínas Proto-Oncogénicas c-akt / Células Endoteliales de la Vena Umbilical Humana / Ondas Ultrasónicas Límite: Humans Idioma: En Revista: Ultrasonics Año: 2022 Tipo del documento: Article País de afiliación: China
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