Study on the preparation of molecular imprinted polymer for analysis of N-phenylglycine in human urine.
J Chromatogr B Analyt Technol Biomed Life Sci
; 1182: 122918, 2021 Oct 01.
Article
en En
| MEDLINE
| ID: mdl-34537499
ABSTRACT
N-phenylglycine (NPG) in human urine could be an important biomarker for predicting cancers, but its detection has difficulty due to its low abundance in urine. Herein, we report a molecular imprinted polymer (MIP) method to efficiently recognize NPG in urine. The MIP was prepared by precipitation polymerization, adopting NPG as the template, acrylamide (AM) as functional monomer, trimethylpropane triacrylate (TRIM) as crosslinking agent, and acetonitrile as porogen. The specificity and selectivity of MIP towards NPG in human urine were determined by comparing MIP's adsorption to the NPG and N-crotonylglycine (NTG) under the same conditions. The result ß = QMIP-NPG/QMIP-NTG = 4.7 indicated the satisfactory specificity and selectivity. Parameters affecting the extraction efficiency were further optimized. Under the optimum conditions, the linear range, limit of detection, and limit of quantification of NPG were 0.5-100 mgâL-1, 1.6 × 10-2 mgâL-1, and 5.5 × 10-2 mgâL-1, respectively. Recoveries of NPG in human urine were in the range of 84.7-100.0% with RSDS of 3.8-10.8%. The developed method demonstrated superior selectivity to the target analyte, which can be applied to separate and enrich the NPG from urine samples.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Impresión Molecular
/
Polímeros Impresos Molecularmente
/
Glicina
Tipo de estudio:
Prognostic_studies
Límite:
Humans
Idioma:
En
Revista:
J Chromatogr B Analyt Technol Biomed Life Sci
Asunto de la revista:
ENGENHARIA BIOMEDICA
Año:
2021
Tipo del documento:
Article
País de afiliación:
China