High-Efficiency Classification of White Blood Cells Based on Object Detection.

ABSTRACT

White blood cells (WBCs) play a significant role in the human immune system, and the content of various subtypes of WBCs is usually maintained within a certain range in the human body, while deviant levels are important warning signs for diseases. Hence, the detection and classification of WBCs is an essential diagnostic technique. However, traditional WBC classification technologies based on image processing usually need to segment the collected target cell images from the background. This preprocessing operation not only increases the workload but also heavily affects the classification quality and efficiency. Therefore, we proposed one high-efficiency object detection technology that combines the segmentation and recognition of targets into one step to realize the detection and classification of WBCs in an image at the same time. Two state-of-the-art object detection models, Faster RCNN and Yolov4, were employed and comparatively studied to classify neutrophils, eosinophils, monocytes, and lymphocytes on a balanced and enhanced Blood Cell Count Dataset (BCCD). Our experimental results showed that the Faster RCNN and Yolov4 based deep transfer learning models achieved classification accuracy rates of 96.25% and 95.75%, respectively. For the one-stage model, Yolov4, while ensuring more than 95% accuracy, its detection speed could reach 60 FPS, which showed better performance compared with the two-stage model, Faster RCNN. The high-efficiency object detection network that does not require cell presegmentation can remove the difficulty of image preprocessing and greatly improve the efficiency of the entire classification task, which provides a potential solution for future real-time point-of-care diagnostic systems.