Development of a pulse-induced electrochemical biosensor based on gluconamide for Gram-negative bacteria detection.
Mikrochim Acta
; 188(11): 399, 2021 Oct 30.
Article
en En
| MEDLINE
| ID: mdl-34716816
Pathogenic bacteria can cause the outbreaks of disease and threaten human health, which stimulates the development of advanced detection techniques. Herein, a specific and sensitive electrochemical biosensor for Gram-negative bacteria was established based on the conductive polymer with artificial muscle properties. The effective recognition was achieved through the specific carbohydrate-carbohydrate interaction between gluconamide and lipopolysaccharide. The application of impulse voltage enhances the efficiency of recognition and shortens the detection time through the temporary deformation of the electrode surface, with a limit of detection (LOD) of 1 × 100 CFU/mL and a linear range of 1 × 100 - 1 × 106 CFU/mL for Escherichia coli (E. coli). In addition to the merits of low cost, high efficiency, and rapidity, the developed label-free electrochemical biosensor can also be applicable for other Gram-negative bacteria, owning promising potential in the application of portable devices and paving a potential way for the construction of electrochemical biosensors.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Contexto en salud:
3_ND
Problema de salud:
3_neglected_diseases
/
3_zoonosis
Asunto principal:
Técnicas Biosensibles
/
Lipopolisacáridos
/
Pseudomonas putida
/
Escherichia coli
/
Gluconatos
Tipo de estudio:
Diagnostic_studies
Límite:
Animals
Idioma:
En
Revista:
Mikrochim Acta
Año:
2021
Tipo del documento:
Article
País de afiliación:
China