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Dietary Nano-ZnO Is Absorbed via Endocytosis and ZIP Pathways, Upregulates Lipogenesis, and Induces Lipotoxicity in the Intestine of Yellow Catfish.
Chen, Shu-Wei; Lv, Wu-Hong; Wu, Kun; Chen, Guang-Hui; Chen, Fang; Song, Chang-Chun; Luo, Zhi.
Afiliación
  • Chen SW; Hubei Hongshan Laboratory, Fishery College, Huazhong Agricultural University, Wuhan 430070, China.
  • Lv WH; Hubei Hongshan Laboratory, Fishery College, Huazhong Agricultural University, Wuhan 430070, China.
  • Wu K; Hubei Hongshan Laboratory, Fishery College, Huazhong Agricultural University, Wuhan 430070, China.
  • Chen GH; Hubei Hongshan Laboratory, Fishery College, Huazhong Agricultural University, Wuhan 430070, China.
  • Chen F; Hubei Hongshan Laboratory, Fishery College, Huazhong Agricultural University, Wuhan 430070, China.
  • Song CC; Hubei Hongshan Laboratory, Fishery College, Huazhong Agricultural University, Wuhan 430070, China.
  • Luo Z; Hubei Hongshan Laboratory, Fishery College, Huazhong Agricultural University, Wuhan 430070, China.
Int J Mol Sci ; 22(21)2021 Nov 07.
Article en En | MEDLINE | ID: mdl-34769475
Nano-sized zinc oxide (nano-ZnO) affects lipid deposition, but its absorption patterns and mechanisms affecting lipid metabolism are still unclear. This study was undertaken to investigate the molecular mechanism of nano-ZnO absorption and its effects on lipid metabolism in the intestinal tissues of a widely distributed freshwater teleost yellow catfish Pelteobagrus fulvidraco. We found that 100 mg/kg dietary nano-ZnO (H-Zn group) significantly increased intestinal Zn contents. The zip6 and zip10 mRNA expression levels were higher in the H-Zn group than those in the control (0 mg/kg nano-ZnO), and zip4 mRNA abundances were higher in the control than those in the L-Zn (50 mg/kg nano-ZnO) and H-Zn groups. Eps15, dynamin1, dynamin2, caveolin1, and caveolin2 mRNA expression levels tended to reduce with dietary nano-ZnO addition. Dietary nano-ZnO increased triglyceride (TG) content and the activities of the lipogenic enzymes glucose 6-phosphate dehydrogenase (G6PD), 6-phosphogluconate dehydrogenase (6PGD), and isocitrate dehydrogenase (ICDH), upregulated the mRNA abundances of lipogenic genes 6pgd, fatty acid synthase (fas), and sterol regulatory element binding protein 1 (srebp1), and reduced the mRNA expression of farnesoid X receptor (fxr) and small heterodimer partner (shp). The SHP protein level in the H-Zn group was lower than that in the control and the L-Zn group markedly. Our in vitro study indicated that the intestinal epithelial cells (IECs) absorbed nano-ZnO via endocytosis, and nano-Zn-induced TG deposition and lipogenesis were partially attributable to the endocytosis of nano-ZnO in IECs. Mechanistically, nano-ZnO-induced TG deposition was closely related to the metal responsive transcription factor 1 (MTF-1)-SHP pathway. Thus, for the first time, we found that the lipogenesis effects of nano-ZnO probably depended on the key gene shp, which is potentially regulated by MTF1 and/or FXR. This novel signaling pathway of MTF-1 through SHP may be relevant to explain the toxic effects and lipotoxicity ascribed to dietary nano-ZnO addition.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Óxido de Zinc / Endocitosis / Lipogénesis / Mucosa Intestinal / Lípidos Límite: Animals Idioma: En Revista: Int J Mol Sci Año: 2021 Tipo del documento: Article País de afiliación: China

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Óxido de Zinc / Endocitosis / Lipogénesis / Mucosa Intestinal / Lípidos Límite: Animals Idioma: En Revista: Int J Mol Sci Año: 2021 Tipo del documento: Article País de afiliación: China
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