A macrophage-T cell coculture model for severe tissue injury-induced T cell death.
STAR Protoc
; 2(4): 100983, 2021 12 17.
Article
en En
| MEDLINE
| ID: mdl-34927092
ABSTRACT
A key observation of tissue injury, such as stroke and burn, is a state of systemic immunosuppression characterized by loss of T cells and rise of infections. Here, we present an in vitro model for cell-cell interactions between innate (macrophages) and adaptive (T cells) immune cells. This protocol facilitates bone marrow-derived macrophages (BMDMs) and splenic T cells in a coculture model. The procedure mimics injury-induced T cell death, which is driven by inflammasome activation in macrophages. For complete details on the use and execution of this protocol, please refer to Roth et al. (2021).
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Linfocitos T
/
Muerte Celular
/
Modelos Inmunológicos
/
Técnicas de Cocultivo
/
Macrófagos
Límite:
Animals
Idioma:
En
Revista:
STAR Protoc
Año:
2021
Tipo del documento:
Article
País de afiliación:
Alemania