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Improved bladder contractility after transplantation of human mesenchymal stem cells overexpressing hepatocyte growth factor into underactive bladder from bladder outlet obstruction models of rats.
Kim, Jae Heon; Yang, Hee Jo; Choi, Sung Sik; Kim, Seung U; Lee, Hong J; Song, Yun Seob.
Afiliación
  • Kim JH; Department of Urology, Soonchunhyang University School of Medicine, Seoul, Republic of Korea.
  • Yang HJ; Department of Urology, Soonchunhyang University School of Medicine, Cheonan, Republic of Korea.
  • Choi SS; Medical Science Research Institute, Chungbuk National University, Cheong Ju, Republic of Korea.
  • Kim SU; Division of Neurology, Department of Medicine, UBC Hospital, University of British Columbia, Vancouver, Canada.
  • Lee HJ; Medical Science Research Institute, Chungbuk National University, Cheong Ju, Republic of Korea.
  • Song YS; Department of Urology, Soonchunhyang University School of Medicine, Seoul, Republic of Korea.
PLoS One ; 16(12): e0261402, 2021.
Article en En | MEDLINE | ID: mdl-34936660
INTRODUCTION: An underactive bladder can lead to difficulty in voiding that causes incomplete emptying of the bladder, suggesting the need for a new strategy to increase bladder contractility in such patients. This study was performed to investigate whether human mesenchymal stem cells (hMSCs) were capable of restoring bladder contractility in rats with underactive bladder due to bladder outlet obstruction (BOO) and enhancing their effects by overexpressing hepatocyte growth factor (HGF) in hMSCs. MATERIALS AND METHODS: The hMSCs were transplanted into the bladder wall of rats. Fifty female Sprague-Dawley rats at six weeks of age were divided into five groups: group 1: control; group 2: sham intervention; group 3: eight-week BOO; group 4: BOO rats transplanted with hMSCs; and group 5: BOO rats transplanted with hMSCs overexpressing HGF. Two weeks after the onset of BOO in groups 4 and 5, hMSCs were injected into the bladder wall. Cystometry evaluation was followed by Masson's trichrome staining of bladder tissues. Realtime PCR and immunohistochemical staining were performed to determine for hypoxia, apoptosis, and angiogenesis. RESULTS: Collagen deposition of bladder increased in BOO but decreased after transplantation of hMSCs. The increased inter-contraction interval and residual urine volume after BOO was reversed after hMSCs transplantation. The decreased maximal voiding pressure after BOO was restored by hMSCs treatment. The mRNA expression of bladder collagen1 and TGF-ß1 increased in BOO but decreased after hMSCs transplantation. The decrease in vWF-positive cells in the bladder following BOO was increased after hMSCs transplantation. Caspase 3 and TUNEL-positive apoptosis of bladder cells increased in BOO but decreased after transplantation of hMSCs. These effects were enhanced by overexpressing HGF in hMSCs. CONCLUSION: Transplantation of hMSCs into bladder wall increased the number of micro-vessels, decreased collagen deposition and apoptosis of detrusor muscle, and improved bladder underactivity. The effects were enhanced by overexpressing HGF in hMSCs. Our findings suggest that the restoration of underactive bladder using hMSCs may be used to rectify micturition disorders in patients following resolution of BOO. Further studies are needed before hMSCs can be used in clinical applications.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Vejiga Urinaria / Obstrucción del Cuello de la Vejiga Urinaria / Factor de Crecimiento de Hepatocito / Trasplante de Células Madre Mesenquimatosas / Vejiga Urinaria de Baja Actividad Tipo de estudio: Prognostic_studies Límite: Animals / Female / Humans Idioma: En Revista: PLoS One Asunto de la revista: CIENCIA / MEDICINA Año: 2021 Tipo del documento: Article

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Vejiga Urinaria / Obstrucción del Cuello de la Vejiga Urinaria / Factor de Crecimiento de Hepatocito / Trasplante de Células Madre Mesenquimatosas / Vejiga Urinaria de Baja Actividad Tipo de estudio: Prognostic_studies Límite: Animals / Female / Humans Idioma: En Revista: PLoS One Asunto de la revista: CIENCIA / MEDICINA Año: 2021 Tipo del documento: Article
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