Monoclonal Antibody-Based Immunosensor for the Electrochemical Detection of Chlortoluron Herbicide in Groundwaters.
Biosensors (Basel)
; 11(12)2021 Dec 13.
Article
en En
| MEDLINE
| ID: mdl-34940271
ABSTRACT
Chlortoluron (3-(3-chloro-p-tolyl)-1,1-dimethyl urea) is an herbicide widely used in substitution to isoproturon to control grass weed in wheat and barley crops. Chlortoluron has been detected in groundwaters for more than 20 years; and dramatic increases in concentrations are observed after intense rain outbreaks. In this context; we developed an immunosensor for the determination of chlortoluron based on competitive binding of specific monoclonal antibodies on chlortoluron and immobilized biotinylated chlortoluron; followed by electrochemical detection on screen-printed carbon electrodes. The optimized immunosensor exhibited a logarithmic response in the range 0.01-10 µg·L-1; with a calculated detection limit (LOD) of 22.4 ng·L-1; which is below the maximum levels allowed by the legislation (0.1 µg·L-1). The immunosensor was used for the determination of chlortoluron in natural groundwaters, showing the absence of matrix effects.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Contexto en salud:
2_ODS3
Problema de salud:
2_quimicos_contaminacion
Asunto principal:
Compuestos de Fenilurea
/
Contaminantes Químicos del Agua
/
Agua Subterránea
/
Técnicas Biosensibles
/
Monitoreo del Ambiente
/
Herbicidas
Tipo de estudio:
Diagnostic_studies
Idioma:
En
Revista:
Biosensors (Basel)
Año:
2021
Tipo del documento:
Article
País de afiliación:
Francia