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Impact of Helicobacter pylori infection on fluid duodenal microbial community structure and microbial metabolic pathways.
Maeda, Tadashi; Zai, Hiroaki; Fukui, Yuto; Kato, Yoshifumi; Kumade, Eri; Watanabe, Toshiyasu; Furusyo, Norihiro; Nakajima, Hitoshi; Arai, Kazuho; Ishii, Yoshikazu; Tateda, Kazuhiro; Urita, Yoshihisa.
Afiliación
  • Maeda T; Department of General Medicine and Emergency Care, Toho University School of Medicine, 6- 11-1 Omori-nishi, Ota-ku, 143-8541, Tokyo, Japan. tadashi.ma@med.toho-u.ac.jp.
  • Zai H; Department of General Medicine and Emergency Care, Toho University School of Medicine, 6- 11-1 Omori-nishi, Ota-ku, 143-8541, Tokyo, Japan.
  • Fukui Y; Department of Microbiology and Infectious Diseases, Toho University School of Medicine, 5- 21-16 Omori-nishi, Ota-ku, 143-8540, Tokyo, Japan.
  • Kato Y; Department of General Medicine, Kyushu University, Fukuoka, Japan.
  • Kumade E; Department of General Medicine and Emergency Care, Toho University School of Medicine, 6- 11-1 Omori-nishi, Ota-ku, 143-8541, Tokyo, Japan.
  • Watanabe T; Department of General Medicine and Emergency Care, Toho University School of Medicine, 6- 11-1 Omori-nishi, Ota-ku, 143-8541, Tokyo, Japan.
  • Furusyo N; Department of General Medicine, Kyushu University, Fukuoka, Japan.
  • Nakajima H; Department of General Medicine and Emergency Care, Toho University School of Medicine, 6- 11-1 Omori-nishi, Ota-ku, 143-8541, Tokyo, Japan.
  • Arai K; Department of General Medicine and Emergency Care, Toho University School of Medicine, 6- 11-1 Omori-nishi, Ota-ku, 143-8541, Tokyo, Japan.
  • Ishii Y; Department of Microbiology and Infectious Diseases, Toho University School of Medicine, 5- 21-16 Omori-nishi, Ota-ku, 143-8540, Tokyo, Japan.
  • Tateda K; Department of Microbiology and Infectious Diseases, Toho University School of Medicine, 5- 21-16 Omori-nishi, Ota-ku, 143-8540, Tokyo, Japan.
  • Urita Y; Department of General Medicine and Emergency Care, Toho University School of Medicine, 6- 11-1 Omori-nishi, Ota-ku, 143-8541, Tokyo, Japan.
BMC Microbiol ; 22(1): 27, 2022 01 15.
Article en En | MEDLINE | ID: mdl-35033024
ABSTRACT

BACKGROUND:

The bioactivities of commensal duodenal microbiota greatly influence the biofunction of hosts. We investigated the role of Helicobacter pylori infection in extra-gastroduodenal diseases by determining the impact of H. pylori infection on the duodenal microbiota. We sequenced 16 S rRNA genes in samples aspirated from the descending duodenum of 47 (male, 20; female, 27) individuals who were screened for gastric cancer. Samples were analysed using 16 S rRNA gene amplicon sequencing, and the LEFSe and Kyoto Encyclopaedia of Genes and Genomes methods were used to determine whether the duodenal microflora and microbial biofunctions were affected using H. pylori infection.

RESULTS:

Thirteen and 34 participants tested positive and negative for H. pylori, respectively. We identified 1,404 bacterial operational taxonomic units from 23 phyla and 253 genera. H. pylori infection changed the relative mean abundance of three phyla (Proteobacteria, Actinobacteria, and TM7) and ten genera (Neisseria, Rothia, TM7-3, Leptotrichia, Lachnospiraceae, Megasphaera, F16, Moryella, Filifactor, and Paludibacter). Microbiota features were significantly influenced in H. pylori-positive participants by 12 taxa mostly classified as Gammaproteobacteria. Microbial functional annotation revealed that H. pylori significantly affected 12 microbial metabolic pathways.

CONCLUSIONS:

H. pylori disrupted normal bacterial communities in the duodenum and changed the biofunctions of commensal microbiota primarily by upregulating specific metabolic pathways. Such upregulation may be involved in the onset of diseases associated with H. pylori infection.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Contexto en salud: 3_ND Problema de salud: 3_zoonosis Asunto principal: Helicobacter pylori / Infecciones por Helicobacter / Duodeno / Redes y Vías Metabólicas / Microbiota / Microbioma Gastrointestinal Límite: Aged / Female / Humans / Male / Middle aged Idioma: En Revista: BMC Microbiol Asunto de la revista: MICROBIOLOGIA Año: 2022 Tipo del documento: Article País de afiliación: Japón

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Contexto en salud: 3_ND Problema de salud: 3_zoonosis Asunto principal: Helicobacter pylori / Infecciones por Helicobacter / Duodeno / Redes y Vías Metabólicas / Microbiota / Microbioma Gastrointestinal Límite: Aged / Female / Humans / Male / Middle aged Idioma: En Revista: BMC Microbiol Asunto de la revista: MICROBIOLOGIA Año: 2022 Tipo del documento: Article País de afiliación: Japón
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