Lysophosphatidylcholine disrupts cell adhesion and induces anoikis in hepatocytes.
FEBS Lett
; 596(4): 510-525, 2022 02.
Article
en En
| MEDLINE
| ID: mdl-35043979
ABSTRACT
Lysophosphatidylcholine (LPC), the active metabolite of palmitate, triggers hepatocyte death by activating endoplasmic reticulum stress and JNK signalling-mediated lipoapoptosis. However, LPC-induced cytotoxicity in hepatocytes is not well understood. Here, we found for the first time that LPC-induced cell rounding occurred prior to apoptosis. LPC-induced rounding of cells reduced both cell-extracellular matrix (ECM) adhesion and cell-cell junctions, which promoted detachment-induced apoptosis (defined as anoikis) in hepatocytes. Further study revealed that LPC altered cellular morphology and cell adhesion by inhibiting integrin and cadherin signalling-mediated microfilament polymerization. We also found that ECM supplementation and microfilament cytoskeletal stabilization inhibited LPC-induced hepatocyte death by attenuating anoikis. Our data indicate a novel cytotoxic process and signalling pathway induced by LPC.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Lisofosfatidilcolinas
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Cadherinas
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Integrinas
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Adhesión Celular
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Anoicis
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Uniones Intercelulares
Idioma:
En
Revista:
FEBS Lett
Año:
2022
Tipo del documento:
Article
País de afiliación:
China