Your browser doesn't support javascript.
loading
[T cell factor 3 (TCF3) is overexpressed in hepatocellular carcinoma and promotes their invasion and metastasis].
Feng, Weibo; Liu, Yao; Chen, Jie; Qiao, Chenyang; Xia, Limin; Wu, Kaichun.
Afiliación
  • Feng W; State Key Laboratory of Cancer Biology, Xijing Hospital of Digestive Disease, The Fourth Military Medical University, Xi'an 710032, China.
  • Liu Y; Department of Cardiology, Hainan Hospital of PLA General Hospital, Sanya 572000, China.
  • Chen J; State Key Laboratory of Cancer Biology, Xijing Hospital of Digestive Disease, The Fourth Military Medical University, Xi'an 710032, China.
  • Qiao C; State Key Laboratory of Cancer Biology, Xijing Hospital of Digestive Disease, The Fourth Military Medical University, Xi'an 710032, China.
  • Xia L; State Key Laboratory of Cancer Biology, Xijing Hospital of Digestive Disease, The Fourth Military Medical University, Xi'an 710032, China.
  • Wu K; State Key Laboratory of Cancer Biology, Xijing Hospital of Digestive Disease, The Fourth Military Medical University, Xi'an 710032, China. *Corresponding author, E-mail: kaicwu@qq.com.
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi ; 38(1): 66-73, 2022 Jan.
Article en Zh | MEDLINE | ID: mdl-35078577
Objective To investigate the expression of T cell factor 3 (TCF3) in hepatocellular carcinoma (HCC), its correlation with the prognosis of HCC patients, and its effect on the invasion, migration, and metastasis of HCC cells. Methods The expression of TCF3 mRNA in HCC tissues was detected with tumor public databases and the expression of TCF3 protein in HCC specimens was detected by immunohistochemical staining. Correlation between TCF3 expression and HCC patients' prognosis was analyzed. Western blot analysis was used to detect the expression of TCF3 in different human HCC cell lines, and lentivirus infection was conducted to construct TCF3-upregulated and TCF3-downregulated HCC cell lines. The effect of TCF3 on the invasion and migration of HCC cells was assessed by in vitro TranswellTM assay, and in vivo intrahepatic tumor implantation models were established to evaluate the effect of TCF3 on the metastatic capacity of HCC cells. Results The expression of TCF3 mRNA was significantly higher in HCC tissues than that in normal liver tissues, and high expression of TCF3 mRNA was closely correlated with decreased overall survival rates of HCC patients. In 120 cases of HCC tissues, the protein level of TCF3 was significantly higher than that in adjacent nontumor tissues, and patients with positive TCF3 expression had a markedly decreased overall survival rate and a higher recurrence rate compared with patients with negative TCF3 expression. In vitro TranswellTM assay indicated that TCF3 upregulation promoted the invasion and migration of PLC/PRF/5 cells, whereas knockdown of TCF3 inhibited the invasion and migration abilities of HCCLM3 cells. Intrahepatic tumor implantation models showed that TCF3 upregulation promoted the metastasis of PLC/PRF/5 cells, while TCF3 knockdown weakened the metastatic capacity of HCCLM3 cells. Conclusion TCF3 expression is significantly upregulated in human HCC tissues, and high TCF3 expression predicts a poor prognosis of HCC patients. TCF3 markedly promotes the invasion, migration, and metastasis of HCC cells.
Asunto(s)
Buscar en Google
Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Carcinoma Hepatocelular / Proteína 1 Similar al Factor de Transcripción 7 / Neoplasias Hepáticas Tipo de estudio: Prognostic_studies Límite: Humans Idioma: Zh Revista: Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi Asunto de la revista: ALERGIA E IMUNOLOGIA Año: 2022 Tipo del documento: Article País de afiliación: China
Buscar en Google
Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Carcinoma Hepatocelular / Proteína 1 Similar al Factor de Transcripción 7 / Neoplasias Hepáticas Tipo de estudio: Prognostic_studies Límite: Humans Idioma: Zh Revista: Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi Asunto de la revista: ALERGIA E IMUNOLOGIA Año: 2022 Tipo del documento: Article País de afiliación: China
...