The Gut Microbiome and Metabolites Are Altered and Interrelated in Patients With Rheumatoid Arthritis.
Front Cell Infect Microbiol
; 11: 763507, 2021.
Article
en En
| MEDLINE
| ID: mdl-35145919
ABSTRACT
The relationship among the gut microbiome, global fecal metabolites and rheumatoid arthritis (RA) has not been systematically evaluated. In this study, we performed 16S rDNA sequencing and liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based nontargeted metabolomic profiling on feces of 26 untreated RA patients and 26 healthy controls. Twenty-six genera and forty-one MS2-identified metabolites were significantly altered in the RA patients. Klebsiella, Escherichia, Eisenbergiella and Flavobacterium were more abundant in the RA patients, while Fusicatenibacter, Megamonas and Enterococcus were more abundant in the healthy controls. Function prediction analysis demonstrated that the biosynthesis pathways of amino acids, such as L-arginine and aromatic amino acids, were depleted in the RA group. In the metabolome results, fecal metabolites including glycerophospholipids (PC(183(9Z,12Z,15Z)/161(9Z)), lysoPE 191, lysoPE 180, lysoPC(180/00)), sphingolipids (Cer(d180/160), Cer(d180/120), Cer(d180/140)), kynurenic acid, xanthurenic acid and 3-hydroxyanthranilic acid were remarkably altered between the RA patients and healthy controls. Dysregulation of pathways, such as tryptophan metabolism, alpha-linolenic acid metabolism and glycerophospholipid metabolism, may contribute to the development of RA. Additionally, we revealed that the gut microbiome and metabolites were interrelated in the RA patients, while Escherichia was the core genus. By depicting the overall landscape of the intestinal microbiome and metabolome in RA patients, our study could provide possible novel research directions regarding RA pathogenesis and targeted therapy.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Contexto en salud:
3_ND
Problema de salud:
3_zoonosis
Asunto principal:
Artritis Reumatoide
/
Microbioma Gastrointestinal
Límite:
Humans
Idioma:
En
Revista:
Front Cell Infect Microbiol
Año:
2021
Tipo del documento:
Article
País de afiliación:
China