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Mitochondrial variant enrichment from high-throughput single-cell RNA sequencing resolves clonal populations.
Miller, Tyler E; Lareau, Caleb A; Verga, Julia A; DePasquale, Erica A K; Liu, Vincent; Ssozi, Daniel; Sandor, Katalin; Yin, Yajie; Ludwig, Leif S; El Farran, Chadi A; Morgan, Duncan M; Satpathy, Ansuman T; Griffin, Gabriel K; Lane, Andrew A; Love, J Christopher; Bernstein, Bradley E; Sankaran, Vijay G; van Galen, Peter.
Afiliación
  • Miller TE; Department of Pathology, Massachusetts General Hospital, Boston, MA, USA.
  • Lareau CA; Broad Institute of MIT and Harvard, Cambridge, MA, USA.
  • Verga JA; Department of Cancer Biology, Dana-Farber Cancer Institute, Boston, MA, USA.
  • DePasquale EAK; Broad Institute of MIT and Harvard, Cambridge, MA, USA.
  • Liu V; Department of Pathology, Stanford University, Stanford, CA, USA.
  • Ssozi D; Department of Genetics, Stanford University, Stanford, CA, USA.
  • Sandor K; Division of Hematology/Oncology, Boston Children's Hospital and Department of Pediatric Oncology, Dana-Farber Cancer Institute, Harvard Medical School, Boston, MA, USA.
  • Yin Y; Broad Institute of MIT and Harvard, Cambridge, MA, USA.
  • Ludwig LS; Department of Cancer Biology, Dana-Farber Cancer Institute, Boston, MA, USA.
  • El Farran CA; Broad Institute of MIT and Harvard, Cambridge, MA, USA.
  • Morgan DM; Division of Hematology, Brigham and Women's Hospital, Department of Medicine, Harvard Medical School, Boston, MA, USA.
  • Satpathy AT; Department of Pathology, Stanford University, Stanford, CA, USA.
  • Griffin GK; Department of Genetics, Stanford University, Stanford, CA, USA.
  • Lane AA; Broad Institute of MIT and Harvard, Cambridge, MA, USA.
  • Love JC; Division of Hematology, Brigham and Women's Hospital, Department of Medicine, Harvard Medical School, Boston, MA, USA.
  • Bernstein BE; Department of Pathology, Stanford University, Stanford, CA, USA.
  • Sankaran VG; Department of Pathology, Stanford University, Stanford, CA, USA.
  • van Galen P; Broad Institute of MIT and Harvard, Cambridge, MA, USA.
Nat Biotechnol ; 40(7): 1030-1034, 2022 07.
Article en En | MEDLINE | ID: mdl-35210612
ABSTRACT
The combination of single-cell transcriptomics with mitochondrial DNA variant detection can be used to establish lineage relationships in primary human cells, but current methods are not scalable to interrogate complex tissues. Here, we combine common 3' single-cell RNA-sequencing protocols with mitochondrial transcriptome enrichment to increase coverage by more than 50-fold, enabling high-confidence mutation detection. The method successfully identifies skewed immune-cell expansions in primary human clonal hematopoiesis.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: ADN Mitocondrial / Secuenciación de Nucleótidos de Alto Rendimiento Límite: Humans Idioma: En Revista: Nat Biotechnol Asunto de la revista: BIOTECNOLOGIA Año: 2022 Tipo del documento: Article País de afiliación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: ADN Mitocondrial / Secuenciación de Nucleótidos de Alto Rendimiento Límite: Humans Idioma: En Revista: Nat Biotechnol Asunto de la revista: BIOTECNOLOGIA Año: 2022 Tipo del documento: Article País de afiliación: Estados Unidos