Rapid cell-free characterization of multi-subunit CRISPR effectors and transposons.

Wimmer, Franziska; Mougiakos, Ioannis; Englert, Frank; Beisel, Chase L

Wimmer, Franziska; Mougiakos, Ioannis; Englert, Frank; Beisel, Chase L.

Afiliación

Wimmer F; Helmholtz Institute for RNA-based Infection Research (HIRI), Helmholtz Centre for Infection Research (HZI), 97080 Würzburg, Germany.
Mougiakos I; Helmholtz Institute for RNA-based Infection Research (HIRI), Helmholtz Centre for Infection Research (HZI), 97080 Würzburg, Germany.
Englert F; Helmholtz Institute for RNA-based Infection Research (HIRI), Helmholtz Centre for Infection Research (HZI), 97080 Würzburg, Germany.
Beisel CL; Helmholtz Institute for RNA-based Infection Research (HIRI), Helmholtz Centre for Infection Research (HZI), 97080 Würzburg, Germany; Medical Faculty, University of Würzburg, 97080 Würzburg, Germany. Electronic address: chase.beisel@helmholtz-hiri.de.

Mol Cell ; 82(6): 1210-1224.e6, 2022 03 17.

Article en En | MEDLINE | ID: mdl-35216669

Asunto(s)

Sistemas CRISPR-Cas; Endonucleasas; Sistema Libre de Células/metabolismo; ADN/genética; Endonucleasas/genética; ARN/metabolismo

Palabras clave

CAST; Cascade; PAM; PAM wheel; PAM-DETECT; TXTL; Type I CRISPR-Cas system; high-throughput screen; restriction enzyme

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Endonucleasas / Sistemas CRISPR-Cas Idioma: En Revista: Mol Cell Asunto de la revista: BIOLOGIA MOLECULAR Año: 2022 Tipo del documento: Article País de afiliación: Alemania

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