Pleomorphic adenoma gene 1 (PLAG1) promotes proliferation and inhibits apoptosis of bovine primary myoblasts through the PI3K-Akt signaling pathway.

Pleomorphic adenoma gene 1 (PLAG1) is a critical candidate gene that affects bovine stature and height. Past studies have focused on correlations between PLAG1 and quantitative traits. However, few studies have focused on studying the function of PLAG1 at the cellular level. Here, we investigated the effects of PLAG1 on bovine primary myoblasts and found it promoted proliferation and inhibited the apoptosis of bovine primary myoblasts. In addition, our study demonstrated that the effects of PLAG1 on myoblast proliferation and apoptosis were through the PI3K-Akt signaling pathway. Further study suggested that there was a potential positive feedback regulation between PLAG1 and PI3K-Akt, and miR-1 acted as a key mediator. Our study provided a theoretical basis for further exploration.