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Distribution of Carbapenemase Genes among Carbapenem-Non-Susceptible Acinetobacter baumanii Blood Isolates in Indonesia: A Multicenter Study.
Anggraini, Dewi; Santosaningsih, Dewi; Saharman, Yulia Rosa; Endraswari, Pepy Dwi; Cahyarini, Cahyarini; Saptawati, Leli; Hayati, Zinatul; Farida, Helmia; Siregar, Cherry; Pasaribu, Munawaroh; Homenta, Heriyannis; Tjoa, Enty; Jasmin, Novira; Sarassari, Rosantia; Setyarini, Wahyu; Hadi, Usman; Kuntaman, Kuntaman.
Afiliación
  • Anggraini D; Doctoral Program, Faculty of Medicine, Universitas Airlangga, Surabaya 60115, Indonesia.
  • Santosaningsih D; Department of Microbiology, Faculty of Medicine, Universitas Riau, Pekanbaru 28133, Indonesia.
  • Saharman YR; Arifin Achmad General Hospital, Pekanbaru 28156, Indonesia.
  • Endraswari PD; Department of Clinical Microbiology, Faculty of Medicine, Universitas Brawijaya, Malang 65145, Indonesia.
  • Cahyarini C; Dr. Saiful Anwar Hospital, Malang 65112, Indonesia.
  • Saptawati L; Department of Clinical Microbiology, Faculty of Medicine, Universitas Indonesia, Jakarta 10320, Indonesia.
  • Hayati Z; Pelni Hospital, Jakarta 11410, Indonesia.
  • Farida H; Department of Medical Microbiology, Faculty of Medicine, Universitas Airlangga, Surabaya 60115, Indonesia.
  • Siregar C; Dr. Soetomo General Academic Hospital, Surabaya 60286, Indonesia.
  • Pasaribu M; Department of Clinical Microbiology, Persahabatan General Hospital, Jakarta 13230, Indonesia.
  • Homenta H; Department of Microbiology, Faculty of Medicine, Universitas Sebelas Maret, Surakarta 57126, Indonesia.
  • Tjoa E; Department of Microbiology, Dr. Moewardi Teaching Hospital, Surakarta 57126, Indonesia.
  • Jasmin N; Department of Microbiology, Faculty of Medicine, Universitas Syiah Kuala, Banda Aceh 23111, Indonesia.
  • Sarassari R; Department of Microbiology, Dr. Zainoel Abidin Hospital, Banda Aceh 24415, Indonesia.
  • Setyarini W; Department of Microbiology, Faculty of Medicine, Diponegoro University, Semarang 50275, Indonesia.
  • Hadi U; Dr. Kariadi Hospital, Semarang 50244, Indonesia.
  • Kuntaman K; H. Adam Malik Hospital, Medan 20136, Indonesia.
Antibiotics (Basel) ; 11(3)2022 Mar 09.
Article en En | MEDLINE | ID: mdl-35326829
ABSTRACT
Carbapenem non-susceptible Acinetobacter baumannii (CNSAB) is an important pathogen that causes nosocomial bacteremia among critically ill patients worldwide. The magnitude of antibiotic resistance of A. baumanii in Indonesia is expected to be significant; however, the data available are limited. The aim of this study was to analyze the genetic profiles of CNSAB isolates from patients with bacteremia in Indonesia. CNSAB isolates from blood cultures of bacteremia patients in 12 hospitals in Indonesia were included. The blood cultures were conducted using the BacT/Alert or BACTEC automated system. The CNSAB were identified with either Vitek 2 system or Phoenix platform followed by a confirmation test using a multiplex polymerase chain reaction (PCR) assay, targeting the specific gyrB gene. The carbapenemase genes were detected by multiplex PCR. In total, 110 CNSAB isolates were collected and were mostly resistant to nearly all antibiotic classes. The majority of CNSAB isolates were susceptible to tigecycline and trimethoprim-sulfamethoxazole (TMP-SMX), 45.5% and 38.2%, respectively. The blaOXA-51-like gene was identified in all CNSAB isolates. Out of the total, 83.6% of CNSAB isolates had blaOXA-23-like gene, 37.3% blaOXA-24-like gene, 4.5% blaNDM-1 gene, 0.9% blaIMP-1 gene, and 0.9% blaVIM gene. No blaOXA-48-like gene was identified. The blaOXA-23-like gene was the predominant gene in all except two hospitals. The presence of the blaOXA-24-like gene was associated with resistance to tigecycline, amikacin, TMP-SMX and cefoperazone-sulbactam, while blaOXA-23-like gene was associated with resistance to TMP-SMX and cefoperazone-sulbactam. In conclusion, the blaOXA-23-like gene was the predominant gene among CNSAB isolates throughout Indonesia. A continuous national surveillance system needs to be established to further monitor the genetic profiles of CNSAB in Indonesia.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Clinical_trials / Prognostic_studies Idioma: En Revista: Antibiotics (Basel) Año: 2022 Tipo del documento: Article País de afiliación: Indonesia

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Clinical_trials / Prognostic_studies Idioma: En Revista: Antibiotics (Basel) Año: 2022 Tipo del documento: Article País de afiliación: Indonesia
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