A novel phenolphthalein-based fluorescent chemosensor for pyrophosphate detection via an Al3+ displacement approach in real samples and living cells.
Spectrochim Acta A Mol Biomol Spectrosc
; 276: 121174, 2022 Aug 05.
Article
en En
| MEDLINE
| ID: mdl-35397452
ABSTRACT
Herein, we report a new phenolphthalein appended Schiff base (PASB) as reversible fluorescent sensor for the detection of pyrophosphate (PPi) ions through the metal displacement mechanism. PASB showed sensing exclusively toward Al3+ ions in DMF/H2O (v/v = 1/4, pH 5.5) solution, which resulted in a significant fluorescence enhancement at 540 nm. The 1 2 binding stoichiometry for the complex formation between PASB and Al3+ was confirmed by Job's plot and mass spectroscopic studies. Moreover, a solution of the in situ formed PASB-Al3+ complex displayed a high selectivity to PPi. The addition of PPi to PASB-Al3+ ensemble significantly quenched its fluorescence. Thus, a dual response was established based on "Off-On-Off" strategy for detection of both Al3+ and PPi. The detection limit is 5.86 nM and 26 nM for Al3+ and PPi, respectively. On this basis, we use PASB to detect Al3+ in food samples. Furthermore, PASB was successfully applicable to detect Al3+ and PPi for intracellular imaging in Human liver cancer cells.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Difosfatos
/
Fenolftaleína
Tipo de estudio:
Diagnostic_studies
Límite:
Humans
Idioma:
En
Revista:
Spectrochim Acta A Mol Biomol Spectrosc
Asunto de la revista:
BIOLOGIA MOLECULAR
Año:
2022
Tipo del documento:
Article
País de afiliación:
China