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MiR-214-3p Prevents the Development of Perioperative Neurocognitive Disorders in Elderly Rats.
Wang, Yu-Hao; Chen, Yong-Wang; Xiao, Wan-Li; Li, Xue-Lian; Feng, Lan; Liu, Yu-Lin; Duan, Xiao-Xia.
Afiliación
  • Wang YH; Department of Anesthesiology, The Affiliated Hospital of Southwest Medical University, Luzhou, 646000, China.
  • Chen YW; Laboratory of Anesthesiology, Southwest Medical University, Luzhou, 646000, China.
  • Xiao WL; Department of Anesthesiology, Southwest Medical University, Luzhou, 646000, China.
  • Li XL; Department of Anesthesiology, The Affiliated Hospital of Southwest Medical University, Luzhou, 646000, China.
  • Feng L; Laboratory of Anesthesiology, Southwest Medical University, Luzhou, 646000, China.
  • Liu YL; Department of Anesthesiology, Southwest Medical University, Luzhou, 646000, China.
  • Duan XX; Department of Anesthesiology, The Affiliated Hospital of Southwest Medical University, Luzhou, 646000, China.
Curr Med Sci ; 42(4): 871-884, 2022 Aug.
Article en En | MEDLINE | ID: mdl-35451808
OBJECTIVE: This study aimed to identify microRNAs (miRNAs) involved in the development of perioperative neurocognitive disorders (PND). METHODS: Plasma exosomal miRNA expression was examined in patients before and after cardiopulmonary bypass (CPB) using microarray and qRT-PCR and these patients were diagnosed as PND later. Elderly rats were subjected to CPB, and the cognitive functions were examined. Bioinformatics analysis was conducted to predict the targets of miR-214-3p. Rats were administered rno-miR-214-3p agomir before or after CPB to investigate the role of miR-214-3p in PND development. RESULTS: We identified 76 differentially expressed plasma exosomal miRNAs in PND patients after surgery (P<0.05, ∣log2FC∣>0.58), including the upregulated hsa-miR-214-3p (P=0.002399392). Prostaglandin-endoperoxide synthase 2 (PTGS2) was predicted as a miR-214-3p target. In rats, CPB reduced the platform crossing numbers and target quadrant stay time, accompanied with hippocampal neuronal necrosis. The rno-miR-214-3p level was significantly increased in plasma exosomes but decreased in rat hippocampus after surgery, exhibiting a negative correlation (P<0.001, r=-0.762). A negative correlation between miR-214-3p and PTGS2 protein expression was also observed in the hippocampus after surgery. Importantly, rno-miR-214-3p agomir treatment, before or after surgery, significantly increased the platform crossing numbers (P=0.035) and target quadrant stay time (P=0.029) compared with negative control. Hippocampal PTGS2 protein level was increased in the untreated surgery group and decreased in response to rno-miR-214-3p agomir treatment before or after surgery (both P<0.05 vs. negative control). CONCLUSION: These data suggest that miR-214-3p/PTGS2 signaling contributes to the development of PND, serving as a potential therapeutic target for PND.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Puente Cardiopulmonar / Trastornos Neurocognitivos / MicroARNs / Exosomas Tipo de estudio: Etiology_studies Límite: Animals / Humans Idioma: En Revista: Curr Med Sci Año: 2022 Tipo del documento: Article País de afiliación: China

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Puente Cardiopulmonar / Trastornos Neurocognitivos / MicroARNs / Exosomas Tipo de estudio: Etiology_studies Límite: Animals / Humans Idioma: En Revista: Curr Med Sci Año: 2022 Tipo del documento: Article País de afiliación: China
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