Micropeptide vsp21 translated by Reovirus circular RNA 000048 attenuates viral replication.
Int J Biol Macromol
; 209(Pt A): 1179-1187, 2022 Jun 01.
Article
en En
| MEDLINE
| ID: mdl-35461859
ABSTRACT
To date, some DNA viruses and single-stranded RNA viruses have been found to generate circRNAs. However, the reports on circRNAs produced by double-stranded RNA viruses are very limited. In this study, Bombyx mori cypovirus (BmCPV), a typical double-stranded RNA virus belonging to the Reoviridae, was demonstrated to generate viral circRNAs (vcircRNAs) and a vcircRNA_000048 whose sequence corresponds with the region 164-1245 nt on the BmCPV genomic dsRNA S5 segment (GQ294468.1) was validated by PCR, Sanger sequencing, reverse transcription-rolling circle amplification, and Northern blotting. Furthermore, we verified that vcircRNA_000048 translates a micropeptide vsp21 with 21 amino acid residues in an IRES-dependent manner, and vsp21 attenuates the viral replication. These findings provided a novel clue to understanding the regulation of viral multiplication and interaction of reovirus with the host.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Reoviridae
/
Bombyx
Límite:
Animals
Idioma:
En
Revista:
Int J Biol Macromol
Año:
2022
Tipo del documento:
Article
País de afiliación:
China