Establishing transgenic murine esophageal organoids.
STAR Protoc
; 3(2): 101317, 2022 06 17.
Article
en En
| MEDLINE
| ID: mdl-35496812
ABSTRACT
Organoid systems are commonly used for disease modeling because of their faithful recapitulation of tissue homeostasis, tissue regeneration, and disease processes. However, there is not an optimal approach for the culture of primary mouse esophageal organoids (EOs). Herein, we provide the detailed steps for an efficient and cost-effective protocol for generating and culturing murine EOs. We also describe how to establish transgenic EOs using viral transduction. For complete details on the use and execution of this protocol, please refer to Zheng et al. (2021).
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Organoides
Límite:
Animals
Idioma:
En
Revista:
STAR Protoc
Año:
2022
Tipo del documento:
Article
País de afiliación:
Estados Unidos