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Diamond Raman laser and Yb fiber amplifier for in vivo multiphoton fluorescence microscopy.
Engelmann, Shaun A; Zhou, Annie; Hassan, Ahmed M; Williamson, Michael R; Jarrett, Jeremy W; Perillo, Evan P; Tomar, Alankrit; Spence, David J; Jones, Theresa A; Dunn, Andrew K.
Afiliación
  • Engelmann SA; Department of Biomedical Engineering, The University of Texas at Austin, 107 W. Dean Keeton, Austin, TX 78712, USA.
  • Zhou A; Department of Biomedical Engineering, The University of Texas at Austin, 107 W. Dean Keeton, Austin, TX 78712, USA.
  • Hassan AM; Department of Biomedical Engineering, The University of Texas at Austin, 107 W. Dean Keeton, Austin, TX 78712, USA.
  • Williamson MR; Institute for Neuroscience, The University of Texas at Austin, 2415 Speedway, Austin, TX 78712, USA.
  • Jarrett JW; Department of Biomedical Engineering, The University of Texas at Austin, 107 W. Dean Keeton, Austin, TX 78712, USA.
  • Perillo EP; Department of Biomedical Engineering, The University of Texas at Austin, 107 W. Dean Keeton, Austin, TX 78712, USA.
  • Tomar A; Department of Biomedical Engineering, The University of Texas at Austin, 107 W. Dean Keeton, Austin, TX 78712, USA.
  • Spence DJ; MQ Photonics, Department of Physics and Astronomy, Macquarie University, NSW 2109, Australia.
  • Jones TA; Institute for Neuroscience, The University of Texas at Austin, 2415 Speedway, Austin, TX 78712, USA.
  • Dunn AK; Department of Biomedical Engineering, The University of Texas at Austin, 107 W. Dean Keeton, Austin, TX 78712, USA.
Biomed Opt Express ; 13(4): 1888-1898, 2022 Apr 01.
Article en En | MEDLINE | ID: mdl-35519268
ABSTRACT
Here we introduce a fiber amplifier and a diamond Raman laser that output high powers (6.5 W, 1.3 W) at valuable wavelengths (1060 nm, 1250 nm) for two-photon excitation of red-shifted fluorophores. These custom excitation sources are both simple to construct and cost-efficient in comparison to similar custom and commercial alternatives. Furthermore, they operate at a repetition rate (80 MHz) that allows fast image acquisition using resonant scanners. With our system we demonstrate compatibility with fast resonant scanning, the ability to acquire neuronal images, and the capability to image vasculature at deep locations (>1 mm) within the mouse cerebral cortex.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Biomed Opt Express Año: 2022 Tipo del documento: Article País de afiliación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Biomed Opt Express Año: 2022 Tipo del documento: Article País de afiliación: Estados Unidos