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Effect of High-intensity Training and Probiotics on Gut Microbiota Diversity in Competitive Swimmers: Randomized Controlled Trial.
Bielik, Viktor; Hric, Ivan; Ugrayová, Simona; Kubánová, Libusa; Putala, Matús; Grznár, Lubos; Penesová, Adela; Havranová, Andrea; Sardzíková, Sára; Grendar, Marián; Baranovicová, Eva; Soltys, Katarína; Kolisek, Martin.
Afiliación
  • Bielik V; Department of Biological and Medical Sciences, Faculty of Physical Education and Sport, Comenius University in Bratislava, 814 69, Bratislava, Slovakia. viktor.bielik@uniba.sk.
  • Hric I; Department of Biological and Medical Sciences, Faculty of Physical Education and Sport, Comenius University in Bratislava, 814 69, Bratislava, Slovakia.
  • Ugrayová S; Department of Biological and Medical Sciences, Faculty of Physical Education and Sport, Comenius University in Bratislava, 814 69, Bratislava, Slovakia.
  • Kubánová L; Department of Biological and Medical Sciences, Faculty of Physical Education and Sport, Comenius University in Bratislava, 814 69, Bratislava, Slovakia.
  • Putala M; Biomedical Center, Institute of Clinical and Translational Research, Slovak Academy of Sciences, 845 05, Bratislava, Slovakia.
  • Grznár L; Department of Outdoor Sports and Swimming, Faculty of Physical Education and Sport, Comenius University in Bratislava, 814 69, Bratislava, Slovakia.
  • Penesová A; Department of Outdoor Sports and Swimming, Faculty of Physical Education and Sport, Comenius University in Bratislava, 814 69, Bratislava, Slovakia.
  • Havranová A; Biomedical Center, Institute of Clinical and Translational Research, Slovak Academy of Sciences, 845 05, Bratislava, Slovakia.
  • Sardzíková S; Biomedical Center, Institute of Clinical and Translational Research, Slovak Academy of Sciences, 845 05, Bratislava, Slovakia.
  • Grendar M; Department of Microbiology and Virology, Faculty of Natural Sciences, Comenius University in Bratislava, 842 15, Bratislava, Slovakia.
  • Baranovicová E; Biomedical Center Martin, Jessenius Faculty of Medicine in Martin, Comenius University in Bratislava, 036 01, Martin, Slovakia.
  • Soltys K; Biomedical Center Martin, Jessenius Faculty of Medicine in Martin, Comenius University in Bratislava, 036 01, Martin, Slovakia.
  • Kolisek M; Department of Microbiology and Virology, Faculty of Natural Sciences, Comenius University in Bratislava, 842 15, Bratislava, Slovakia.
Sports Med Open ; 8(1): 64, 2022 May 10.
Article en En | MEDLINE | ID: mdl-35536489
ABSTRACT

BACKGROUND:

Physical exercise has favorable effects on the structure of gut microbiota and metabolite production in sedentary subjects. However, little is known whether adjustments in an athletic program impact overall changes of gut microbiome in high-level athletes. We therefore characterized fecal microbiota and serum metabolites in response to a 7-week, high-intensity training program and consumption of probiotic Bryndza cheese.

METHODS:

Fecal and blood samples and training logs were collected from young competitive male (n = 17) and female (n = 7) swimmers. Fecal microbiota were categorized using specific primers targeting the V1-V3 region of 16S rDNA, and serum metabolites were characterized by NMR-spectroscopic analysis and by multivariate statistical analysis, Spearman rank correlations, and Random Forest models.

RESULTS:

We found higher α-diversity, represented by the Shannon index value (HITB-pre 5.9 [± 0.4]; HITB-post 6.4 [± 0.4], p = 0.007), (HIT-pre 5.5 [± 0.6]; HIT-post 5.9 [± 0.6], p = 0.015), after the end of the training program in both groups independently of Bryndza cheese consumption. However, Lactococcus spp. increased in both groups, with a higher effect in the Bryndza cheese consumers (HITB-pre 0.0021 [± 0.0055]; HITB-post 0.0268 [± 0.0542], p = 0.008), (HIT-pre 0.0014 [± 0.0036]; HIT-post 0.0068 [± 0.0095], p = 0.046). Concomitant with the increase of high-intensity exercise and the resulting increase of anaerobic metabolism proportion, pyruvate (p[HITB] = 0.003; p[HIT] = 0.000) and lactate (p[HITB] = 0.000; p[HIT] = 0.030) increased, whereas acetate (p[HITB] = 0.000; p[HIT] = 0.002) and butyrate (p[HITB] = 0.091; p[HIT] = 0.019) significantly decreased.

CONCLUSIONS:

Together, these data demonstrate a significant effect of high-intensity training (HIT) on both gut microbiota composition and serum energy metabolites. Thus, the combination of intensive athletic training with the use of natural probiotics is beneficial because of the increase in the relative abundance of lactic acid bacteria.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Clinical_trials Idioma: En Revista: Sports Med Open Año: 2022 Tipo del documento: Article País de afiliación: Eslovaquia

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Clinical_trials Idioma: En Revista: Sports Med Open Año: 2022 Tipo del documento: Article País de afiliación: Eslovaquia
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