Bacterial Infection Induces Ultrastructural and Transcriptional Changes in the King Oyster Mushroom (Pleurotus eryngii).

ABSTRACT

Pleurotus eryngii (king oyster mushroom) is a commercially important mushroom with high nutritional and economic value. However, soft rot disease, caused by the pathogenic bacterium Erwinia beijingensis, poses a threat to its quality and production. Morphological and ultrastructural observations of P. eryngii were conducted at early, middle, and late stages of infection. At 2 days postinoculation (dpi), small yellow spots on the fruiting body were observed. The infected tissue displayed hyphal deformations and breaks at 5 dpi. At 9 dpi, damage to cell wall integrity and absence of intact cellular organelles were observed and the diseased fruiting bodies were unable to grow normally. Transcriptome analysis identified 4,296 differentially expressed genes in the fruiting body following infection. In fact, broad transcriptional reprogramming was observed in infected fruiting bodies compared to controls. The affected pathways included antioxidant systems, peroxisome biogenesis, autophagy, and oxidation-reduction. More specifically, pex genes were downregulated during infection, indicating impaired peroxisome homeostasis and redox balance. Additionally, genes encoding chitinase, ß-1,3-glucanase, and proteases associated with cell wall degradation were upregulated in infected P. eryngii. This study provides insights into the responses of P. eryngii during soft rot disease and facilitates the understanding of the pathogenic process of bacteriosis in mushrooms. IMPORTANCEPleurotus eryngii (king oyster mushroom) is a popular and economically valuable edible mushroom; however, it suffers from various bacterial diseases, including soft rot disease caused by the bacterium Erwinia beijingensis. Here, we examined bacterial infection of the mushroom through morphological and ultrastructural observations as well as transcriptome analysis. Pathogen attack damaged the cell structure of P. eryngii, including the cell wall, and also induced high levels of reactive oxygen species. These results were reflected in differential gene expression in P. eryngii as a response to the pathogenic bacteria, including genes involved in antioxidant systems, peroxisome biogenesis, autophagy, oxidation-reduction, ribosome biogenesis, and cell-wall degradation, among others. This study provides insights into the structural and molecular responses of P. eryngii during soft rot disease, improving our understanding and the potential control of the pathogenic process of bacteriosis in mushrooms.