Artifacts in optical projection tomography due to refractive-index mismatch: model and correction.
Opt Lett
; 47(11): 2618-2621, 2022 Jun 01.
Article
en En
| MEDLINE
| ID: mdl-35648888
ABSTRACT
Optical projection tomography (OPT) is a powerful tool for three-dimensional (3D) imaging of mesoscopic samples. While it is able to achieve resolution of a few tens of microns over a sample volume of several cubic centimeters, the reconstructed images often suffer from artifacts caused by inaccurate calibration. In this work, we focus on the refractive-index mismatch between the sample and the surrounding medium. We derive a 3D cone-beam forward model of OPT that approximates the effect of refractive-index mismatch. We then implement a fast and efficient reconstruction method to correct for the induced seagull-shaped artifacts on experimental images of fluorescent beads.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Artefactos
/
Tomografía Óptica
Idioma:
En
Revista:
Opt Lett
Año:
2022
Tipo del documento:
Article