Sorption and degradation of prothioconazole and its metabolites in soils and water sediments, and its combinative toxicity to Gobiocypris rarus.

To reduce detrimental effects to the environment, the application of prothioconazole and its metabolites requires comprehensive evaluation, which has been dine for the first time in this study. The behavior of prothioconazole, including degradation and sorption under aerobic and anaerobic conditions, was evaluated in three common soil types and two types of water-sediment systems under different environmental conditions. Individual and joint toxicities of prothioconazole and its metabolites, M01 and M04, on aquatic organisms, including the Gobiocypris rarus, are also investigated in the present study. Under aerobic and anaerobic conditions, the half-life of prothioconazole in the three types of soils ranged from 0.0565 to 2.27 days and 0.138-1.73 days, respectively. Under aerobic conditions, the half-life of prothioconazole in the Hunan paddy area and Beijing Qidu reservoir water-sediment samples were 2.18 and 1.58 days, respectively. In soil and water-sediment samples, prothioconazole degraded to M01 and M04, and the formation rate of M04 was higher than M01 under aerobic condition. M04 and M01 gradually increased to a peak value in soil and water-sediment systems, then decreased over time, while prothioconazole gradually decreased. The half-life of prothioconazole in soils was lower than its metabolites, with the DT50 of metabolites ranging from 16.6 to 99.6 days, 15.8 and 50.7 days for M01 and M04 under aerobic condition, respectively. While the adsorption capacities (Kf values) of M04 and M01 ranged from 2.09 to 88.92 and 8.98 to 243.30 (µg/g)/(mg/L), respectively, in the three soils. Regarding toxicity to aquatic organisms, the metabolites did not show higher toxicity than prothioconazole, except M01 on Gobiocypris rarus. Joint toxicity assays showed that mixtures of prothioconazole with its metabolites exhibited higher toxicity than any compound individually and indicated synergistic interactions could occur at equitoxic ratios and equivalent concentrations. This study provides a comprehensive investigation on the fate and environmental risk posed by prothioconazole.