Cryptosporidium diagnosis in different groups of children and characterization of parasite species.
Rev Soc Bras Med Trop
; 55: e00412022, 2022.
Article
en En
| MEDLINE
| ID: mdl-35894396
ABSTRACT
BACKGROUND:
Microscopy and enzyme-linked immunosorbent assay (ELISA) are routinely used for Cryptosporidium diagnosis, without differentiating the parasite species.METHODS:
Children's feces were analyzed by modiï¬ed Ziehl-Neelsen (mZN) and ELISA for Cryptosporidium diagnosis and by polymerase chain reaction-restriction fragment length polymorphism for species identification.RESULTS:
Cryptosporidium frequency was 2.6%. The sensitivity and specificity of ELISA were 85.7% and 99.7%, respectively, with excellent concordance with mZN (kappa=0.854). Parasite species were characterized as Cryptosporidium hominis (78.3%), Cryptosporidium felis (17.4%), and Cryptosporidium parvum (4.3%).CONCLUSIONS:
Coproantigen ELISA is as efficient as mZN for Cryptosporidium diagnosis. Cryptosporidium genotyping suggests anthroponotic and zoonotic transmission to children.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Contexto en salud:
3_ND
Problema de salud:
3_neglected_diseases
/
3_zoonosis
Asunto principal:
Cryptosporidium parvum
/
Criptosporidiosis
/
Cryptosporidium
Tipo de estudio:
Diagnostic_studies
/
Prognostic_studies
Límite:
Child
/
Humans
Idioma:
En
Revista:
Rev Soc Bras Med Trop
Año:
2022
Tipo del documento:
Article
País de afiliación:
Brasil