A modified CUT&RUN-seq technique for qPCR analysis of chromatin-protein interactions.
STAR Protoc
; 3(3): 101529, 2022 09 16.
Article
en En
| MEDLINE
| ID: mdl-35928003
ABSTRACT
Chromatin immunoprecipitation coupled with quantitative PCR (ChIP-qPCR) even with optimization may give low signal-to-background ratio and spatial resolution. Here, we adapted Cleavage Under Targets and Release Using Nuclease (CUT&RUN) (originally developed by the Henikoff group) to develop CUT&RUN-qPCR. By studying the recruitment of selected proteins (but amenable to other proteins), we find that CUT&RUN-qPCR is more sensitive and gives better spatial resolution than ChIP-qPCR. For complete details on the use and execution of this protocol, please refer to Skene et al. (2018) and Skene and Henikoff (2017).
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Cromatina
/
Cromosomas
Idioma:
En
Revista:
STAR Protoc
Año:
2022
Tipo del documento:
Article